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Advanced Protocols in Oxidative Stress II pp 123–128Cite as

Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 594))

Abstract

Erythrocytes are readily available cells and a good model system to study the health status of individuals with pathologic complications. It can also serve as a meaningful target to study toxicant/xenobiotic-induced damages. We have prepared different concentrations of a carbamate pesticide (carbofuran) and carried out experiments to determine its toxicity on erythrocytes in terms of mean erythrocyte fragility (MEF). We observed a significant alteration in the osmotic fragility upon treatment with carbofuran. In our earlier studies we have observed a good correlation between OF and OS in diabetic subjects. Study reveals OF as a potential biomarker of oxidative membrane damage in pathologic conditions as well as toxicant/xenobiotic/pesticide-induced oxidative membrane damage to erythrocytes.

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Acknowledgments

DKR and PKR are thankful to CSIR and ICMR, respectively, for the award of SRF to them.

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Authors and Affiliations

  1. Medicinal Research Lab, Drug Discovery & Development Division, Alternative Therapeutics Unit, Department of Chemistry, University of Allahabad, Allahabad, India

    Bechan Sharma & Devendra K. Rai

  2. Department of Biochemistry, University of Allahabad, Allahabad, India

    Prashant Kumar Rai, S. I. Rizvi & Geeta Watal

Authors
  1. Bechan Sharma
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  2. Devendra K. Rai
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  3. Prashant Kumar Rai
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  4. S. I. Rizvi
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  5. Geeta Watal
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Corresponding author

Correspondence to Bechan Sharma .

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Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, USA

    Donald Armstrong

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Sharma, B., Rai, D.K., Rai, P.K., Rizvi, S.I., Watal, G. (2010). Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology, vol 594. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-411-1_8

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  • DOI: https://doi.org/10.1007/978-1-60761-411-1_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-410-4

  • Online ISBN: 978-1-60761-411-1

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