Abstract
Detailed practical information is provided with emphasis on mapping cytosolic and mitochondrial pH, mitochondrial Na+, and briefly also aspects related to mitochondrial Ca2+ measurements in living cells, as grown on (un)coated glass coverslips. This chapter lists (laser scanning confocal) microscope instrumentation and setup requirements for proper imaging conditions, cell holders, and an easy-to-use incubator stage. For the daily routine of preparing buffer and calibration solutions, extensive annotated protocols are provided. In addition, detailed measurement and image analysis protocols are given to routinely obtain optimum results with confidence, while avoiding a number of typical pitfalls.
The authors Martin vandeVen and Corina Balut have equally contributed
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Notes
- 1.
The authors Martin vandeVen and Corina Balut have equally contributed
References
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vandeVen, M., Balut, C., Baron, S., Smets, I., Steels, P., Ameloot, M. (2010). Analysis of Mitochondrial pH and Ion Concentrations. In: Papkovsky, D. (eds) Live Cell Imaging. Methods in Molecular Biology, vol 591. Humana Press. https://doi.org/10.1007/978-1-60761-404-3_17
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DOI: https://doi.org/10.1007/978-1-60761-404-3_17
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