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Parasite Challenge as Host Resistance Models for Immunotoxicity Testing

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Immunotoxicity Testing

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 598))

Abstract

Identification of potentially immunosuppressive compounds typically involves assessing a combination of observational endpoints as surrogates for functional endpoints and functional endpoints as surrogates for resistance to infectious or neoplastic disease. Host resistance assays are considered to be the “gold standard” against which suppression of immune function at the molecular or cellular level can be judged, because resistance to infection, regardless of the actual pathogen, involves multiple pathways of effector function to neutralize or eliminate pathogens. Resistance to infection with the parasitic nematode Trichinella spiralis has been used to assess immune function following exposure to a variety of immunotoxicants at the whole animal level. The various immunological mechanisms that are responsible for resistance to different phases of the life cycle are well documented, as are the effects of immunosuppression on the outcome of infection. This chapter describes methods to assess elimination of adult parasites from the small intestine, body burdens of larvae, as well as antibody responses and lymphocyte responses to parasite antigens

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Acknowledgments

Thanks to Mr. Carey Copeland and Ms. Debbie Andrews for excellent technical assistance in the development of the protocols, and to Drs. Christal Bowman and Marsha Ward for helpful comments and suggestions on the manuscript.

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Authors and Affiliations

  1. Immunotoxicology Branch, Division of National Health and Environmental Effects, United State Environmental Protection Agency, Research Triangle Park, Durham, NC, USA

    Robert W. Luebke

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  1. Robert W. Luebke
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Correspondence to Robert W. Luebke .

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Editors and Affiliations

  1. College of Veterinary Medicine, Cornell University, Ithaca, 14853, U.S.A.

    Rodney R. Dietert

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Luebke, R.W. (2010). Parasite Challenge as Host Resistance Models for Immunotoxicity Testing. In: Dietert, R. (eds) Immunotoxicity Testing. Methods in Molecular Biology™, vol 598. Humana Press. https://doi.org/10.1007/978-1-60761-401-2_9

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  • DOI: https://doi.org/10.1007/978-1-60761-401-2_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-400-5

  • Online ISBN: 978-1-60761-401-2

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