Abstract
Biological risk management is required in modern tissue engineering. Particular attention should be paid to the culture medium and the scaffold used. In this perspective, it is important to define minimal culture conditions which allow proper growth and differentiation of epithelial cells in vitro. We propose a simple experimental system which permits the generation of three-dimensional epidermal constructs using a collagen layer as a scaffold mimicking the entire dermal tissue and without the need of any feeder layer. Although showing significant differences compared to natural epidermis, these epidermal constructs appear useful to study keratinocyte differentiation and epidermis histogenesis.
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Acknowledgments
We are grateful to Dr. Giuliano Mazzini (Institute of Molecular Genetics IGM-CNR, Histochemistry and Cytometry Unit, Pavia) and to Dr. Patrizia Vaghi (Centro Grandi Strumenti, University of Pavia) for precious helpful discussion and technical assistance with flow cytometry and laser scanning confocal microscopy analyses. The authors are also grateful to Prof. Angela Faga and Dr. Emanuele Nespoli (Dept. of Plastic surgery, University of Pavia) for kindly providing human skin biopsies. We thank Mrs Aurora Farina (Dept. of Experimental Medicine, Histology and Embryology Unit, University of Pavia) for valuable technical help and assistance with immunohistochemistry. This research was supported by grants from the University of Pavia (FAR) and Banca del Monte di Lombardia Foundation.
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Riva, F., Casasco, A., Casasco, M., Calligaro, A., Cornaglia, A.I. (2010). Growth and Stratification of Epithelial Cells in Minimal Culture Conditions. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 585. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-380-0_3
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DOI: https://doi.org/10.1007/978-1-60761-380-0_3
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