Summary
Methods for using non-ionic detergents to produce demembranated and reactivated cilia and flagella are described in detail. Demembranated and reactivated cell models are useful as a research tool for studying motility function in flagella and cilia. When the plasma membrane is removed, the factors regulating motility can be studied under standardized experimental conditions that otherwise would be impossible. Practical insight is provided to understand the important factors in producing stable reactivated models. In addition, several useful variations of the method are presented for different types of mammalian and non-mammalian flagellar and ciliary systems.
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Acknowledgments
Thanks to Danial J. Oberski for testing the adaptation of the method to Chlamydomonas, Lisa J. Macauley for optimizing the mouse sperm reactivation protocol and Kristen C. Ponichter for refining the method of mouse sperm axoneme disintegration. Supported by N.S.F. Grant MCB-0516181.
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Lindemann, C.B., Lesich, K.A. (2009). Detergent-Extracted Models for the Study of Cilia or Flagella. In: Gavin, R. (eds) Cytoskeleton Methods and Protocols. Methods in Molecular Biology, vol 586. Humana Press. https://doi.org/10.1007/978-1-60761-376-3_19
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DOI: https://doi.org/10.1007/978-1-60761-376-3_19
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