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Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro

  • Cécile Breyton
  • Bernard Pucci
  • Jean-Luc PopotEmail author
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 601)

Abstract

Handling integral membrane proteins in aqueous solutions traditionally relies on the use of detergents, which are surfactants capable of dispersing the components of biological membranes into mixed micelles. The dissociating character of detergents, however, most often causes solubilized membrane proteins to be unstable. This has prompted the development of alternative, less-aggressive surfactants designed to keep membrane proteins soluble, after they have been solubilized, under milder conditions. A short overview is presented of the structure, properties, and uses of two families of such surfactants: amphiphilic polymers (“amphipols”) and fluorinated surfactants.

Key words

Amphipols detergents fluorinated surfactants membrane proteins 

Notes

Acknowledgments

Particular thanks are due to J.-L. Banères, L.J. Catoire, D. Charvolin, T. Dahmane, F. Giusti, Y. Gohon, F. Lebaupain, K.L. Martinez, and M. Zoonens for suggestions about the manuscript and help with the figures. Thanks are also due to A. Polidori and G. Durand, from LCBOSMV, who were greatly involved in the synthesis and physical-chemical analysis of various FSs or non-ionic APols. This work has benefited, in particular, from funding by the EC BIO4-CT98-0269; STREP LSHG-CT-2005-513770 IMPS (Innovative Tools for Membrane Protein Structural Proteomics), the Human Frontier Science Program Organization (grant RG00223/2000-M), the Agence Nationale pour la Recherche PCV07 186241, and the CNRS (Interdisciplinary program Physique et Chimie du Vivant; DRITT grant ST 83747-04).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Cécile Breyton
    • 1
  • Bernard Pucci
    • 2
  • Jean-Luc Popot
    • 3
    Email author
  1. 1.Laboratoire de Physico-Chimie Moléculaire des Membranes Biologiques and Université Paris-7, UMR 5075 CNRS/CEA/UJFGrenobleFrance
  2. 2.Laboratoire de Chimie Bioorganique et des Systèmes Moléculaires VectorielsUniversité d’Avignon et des Pays de Vaucluse, Faculté des SciencesAvignonFrance
  3. 3.Laboratoire de Physico-Chimie Moléculaire des Membranes BiologiquesCNRS and Université Paris-7, Institut de Biologie Physico-ChimiqueParisFrance

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