Abstract
Posttranslational protein modification by the Small Ubiquitin-like MOdifiers (SUMO) is involved in many cellular functions including organization of nuclear structures and chromatin, transcriptional regulation, and nucleo-cytoplasmic transport. Both genetic and biochemical studies indicate that the SUMO modification pathway plays an important role in proper cell cycle control, especially in the normal progression of mitosis. DNA topoisomerase II has been shown to be modified by SUMO in budding yeast as well as in vertebrates. We have shown by biochemical analysis using the Xenopus egg extract (XEE) cell-free assay system that DNA topoisomerase IIα (Topo IIα) is modified by SUMO-2/3 on mitotic chromosomes in the early stages of mitosis. Inhibition of mitotic SUMOylation in the XEE assay system causes aberrant sister chromatid separation in anaphase and alters Topo IIα association with chromosomes.
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Acknowledgments
I would like to thank to Mary Dasso for critical reading and her support. This work was supported by NIH/NCRR, CCET-COBRE (P20 RR015563), and is currently supported by NIH/NIGMS (GM080278).
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Azuma, Y. (2009). Analysis of SUMOylation of Topoisomerase IIα with Xenopus Egg Extracts. In: Clarke, D. (eds) DNA Topoisomerases. Methods in Molecular Biology™, vol 582. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-340-4_17
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DOI: https://doi.org/10.1007/978-1-60761-340-4_17
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