Skip to main content
SpringerLink
Log in

Assessing the Neuroprotective Effect of Antioxidative Food Factors by Application of Lipid-Derived Dopamine Modification Adducts

  • Protocol
  • First Online:
Lipidomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 580))

Summary

Advances in understanding the neurodegenerative pathologies are creating new opportunities for the development of neuroprotective therapies, such as antioxidant food factors, lifestyle modification, and drugs. However, the biomarker by which to determine the effect of the agent on neurodegeneration is limited. We here address hexanoyl dopamine (HED), one of novel dopamine adducts derived from brain polyunsaturated acid, referring to its in vitro formation, potent toxicity to SH-SY5Y cells, and application to assess the neuroprotective effect of antioxidative food factors. Dopamine is a neurotransmitter and its deficiency is a characterized feature in Parkinson’s disease (PD), thereby HED represents a new addition to understanding of dopamine biology and pathophysiology of PD and a novel biomarker for the assessment of neuroprotective therapies. We have established an analytical system using for the detection of HED and its toxicity to the neuroblstoma cell line, SH-SY5Y cells. Here, we discuss the characteristics of the system and its applications to investigate the neuroprotective effect of several antioxidants that originate from food.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Galvan, A. and Wichmann, T. (2008) Pathophysiology of parkinsonism. Clin Neurophysiol. 119(7), 1459–1474

    Article  CAS  PubMed  Google Scholar 

  2. Jenner, P. (2003) Oxidative stress in Parkinson’s disease.Ann. Neurol. 53(Suppl 3), S26–S38

    Article  CAS  PubMed  Google Scholar 

  3. Porter, N.A., Caldwell, S.E., and Mills, K.A. (1995) Mechanisms of free radical oxidation of unsaturated lipids. Lipids 30(4), 277–290.

    Article  CAS  PubMed  Google Scholar 

  4. Kato, Y., Makino, Y., and Osawa, T. (1997) Characterization of a specific polyclonal antibody against 13-hydroperoxyoctadecadienoic acid-modified protein: formation of lipid hydroperoxide-modified apoB-100 in oxidized LDL. J. Lipid Res. 38(7), 1334–1346.

    CAS  PubMed  Google Scholar 

  5. Kato, Y. and Osawa, T. (1998) Detection of oxidized phospholipid-protein adducts using anti-15-hydroperoxyeicosatetraenoic acid-modified protein antibody: contribution of esterified fatty acid-protein adduct to oxidative modification of LDL. Arch. Biochem. Biophys. 351(1), 106–114.

    Article  CAS  PubMed  Google Scholar 

  6. Kato, Y., Mori, Y., Makino, Y., Morimitsu, Y., Hiroi, S., Ishikawa, T., and Osawa, T. (1999) Formation of Nepsilon-(hexanonyl)lysine in protein exposed to lipid hydroperoxide. A plausible marker for lipid hydroperoxide-derived protein modification. J. Biol. Chem. 274(29), 20406–20414.

    Article  CAS  PubMed  Google Scholar 

  7. Kawai, Y., Kato, Y., Fujii, H., Mkino, Y., Mori, Y., Naito, N., and Osawa, T. (2003) Immunochemical detection of a novel lysine adduct using an antibody to linoleic acid hydroperoxide-modified protein. J. Lipid Res. 44(6), 1124–1131

    Article  CAS  PubMed  Google Scholar 

  8. Kawai, Y., Fujii, H., Kato, Y., Kodama, M., Naito, N., Uchida, K., and Osawa, T. (2004) Esterified lipid hydroperoxide-derived modification of protein: formation of a carboxyalkylamide-type lysine adduct in human atherosclerotic lesions. Biochem. Biophys. Res. Commun. 313(2), 271–276

    Article  CAS  PubMed  Google Scholar 

  9. Kawai, Y., Fujii, H., Okada, M., Tsuchie, Y., Uchida, K., and Osawa, T. (2006) Formation of Nepsilon-(succinyl)lysine in vivo: a novel marker for docosahexaenoic acid-derived protein modification. J. Lipid Res. 47(7), 1386–1398

    Article  CAS  PubMed  Google Scholar 

  10. Bove, J., Prou, D., Perier, C., and Przedborski, S. (2005) Toxin-induced models of Parkinson’s disease. NeuroRx 2(3), 484–494

    Article  PubMed  Google Scholar 

  11. Gotz, M.E., Kunig, G., Riederer, P., and Youdim, M.B. (1994) Oxidative stress: free radical production in neural degeneration. Pharmacol. Ther. 63(1), 37–122

    Article  CAS  PubMed  Google Scholar 

  12. Hald, A. and Lotharius, J. (2005) Oxidative stress and inflammation in Parkinson’s disease: is there a causal link? Exp. Neurol. 193(2), 279–290

    Article  CAS  PubMed  Google Scholar 

  13. LaVoie, M.J. and Hastings, T.G. (1999) Peroxynitrite- and nitrite-induced oxidation of dopamine: implications for nitric oxide in dopaminergic cell loss. J. Neurochem. 73(6), 2546–2554

    Article  CAS  PubMed  Google Scholar 

  14. Yamagishi, M., Osakab, N., Takizawa, T., and Osawa, T. (2001) Cacao liquor polyphenols reduce oxidative stress without maintaining alpha-tocopherol levels in rats fed a vitamin E-deficient diet. Lipids 36(1), 67–71

    Article  CAS  PubMed  Google Scholar 

  15. Atkinson, J., Epand, R.F., and Epand, R.M. (2008) Tocopherols and tocotrienols in membranes: a critical review. Free Radic Biol Med. 44 (5), 739–764

    Article  CAS  PubMed  Google Scholar 

  16. Osawa, T. and Kato, Y. (2005) Protective role of antioxidative food factors in oxidative stress caused by hyperglycemia. Ann. N.Y. Acad. Sci. 1043, 440–451

    Article  CAS  PubMed  Google Scholar 

  17. Kitani K., Osawa T., and Yokozawa T. (2007) The effects of tetrahydrocurcumin and green tea polyphenol on the survival of male C57BL/6 mice. Biogerontology 8(5), 567–573

    Article  CAS  PubMed  Google Scholar 

  18. Yokota, T., Matsuzaki, Y., Koyama, M., Hitomi, T., Kawanaka, M., Enoki-Konishi, M., Okuyama, Y., Takayasu, J., Nishino, H., Nishikawa, A., Osawa T, and Sakai T. (2007) Sesamin, a lignan of sesame, down-regulates cyclin D1 protein expression in human tumor cells. Cancer Sci. 98(9), 1447–1453

    Article  CAS  PubMed  Google Scholar 

  19. Sheng HQ, Hirose Y, Hata K, Zheng Q, Kuno T, Asano A, Yamada Y, Hara A, Osawa T, and Mori H. (2007) Modifying effect of dietary sesaminol glucosides on the formation of azoxymethane-induced premalignant lesions of rat colon. Cancer Lett. 246, 63–68

    Article  CAS  PubMed  Google Scholar 

  20. Miyake, Y., Fukumoto, S., Okada, M., Sakaida, K., Nakamura, Y., and Osawa, T. (2005) Antioxidative catechol lignans converted from sesaminol triglucoside by culturing with Aspergillus. J. Agric.Food Chem. 53(1), 22–27.

    Article  CAS  PubMed  Google Scholar 

  21. Kang, M.-H., Naito, M., Sakai, K., Uchida, K., and Osawa, T. (2000) Mode of action of sesame lignans in protecting low-density lipoprotein against oxidative damage in vitro. Life Sciences 66, 161–171.

    Article  CAS  PubMed  Google Scholar 

  22. Liu, X., and Osawa, T. (2007) Cis astaxanthin and especially 9-cis astaxanthin exhibits a higher antioxidant activity in vitro compared to the all-trans isomer. Biochem. Biophys. Res. Commun. 357, 187–193

    Article  CAS  PubMed  Google Scholar 

  23. Aoi, W., Naito, Y., Takanami, Y., Ishii, T., Kawai, Y., Akagiri, S., Kato, Y., Osawa, T., and Yoshikawa, T. (2008) Astaxanthin improves muscle lipid metabolism in exercise via inhibitory effect of oxidative CPT I modification. Biochem. Biophys. Res. Comn. 366, 892–897.

    Article  CAS  Google Scholar 

  24. Liu, X. B., Shibata, T., Hisaka, S., and Osawa, T. (2008) DHA hydroperoxides as a potential inducer of neuronal cell death: a mitochondrial dysfunction-mediated pathway. J. Clin. Biochem. Nutr. 43(1), 26–33

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Chikusa, Japan

    Xuebo Liu, Naruomi Yamada & Toshihiko Osawa

Authors
  1. Xuebo Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naruomi Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshihiko Osawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, U.S.A.

    Donald Armstrong

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Liu, X., Yamada, N., Osawa, T. (2009). Assessing the Neuroprotective Effect of Antioxidative Food Factors by Application of Lipid-Derived Dopamine Modification Adducts. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology™, vol 580. Humana Press. https://doi.org/10.1007/978-1-60761-325-1_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-60761-325-1_8

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-324-4

  • Online ISBN: 978-1-60761-325-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation