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Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography, HPLC, and On-line MS

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Lipidomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 580))

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Summary

Various analytical techniques have been adopted for the isolation and identification of the oxolipids and for determining their functionality. Gas chromatography in combination with mass spectrometry (MS) has been specifically utilized in analysis of isoprostanes and other low molecular weight oxolipids, although it requires derivatization of the solutes. In contrast, liquid chromatography (LC) in combination with on-line MS has proven to be well suited for analysis of intact oxolipids without (or minimal) derivatization. LC-MS has also been helpful for the identification of lipidomic changes resulting from covalent binding of lipid ester core aldehydes to amino lipids, amino acids, peptides, and proteins. This chapter reviews the use of the above techniques for lipidomic analysis of the autoxidation products of cholesteryl esters and glycerolipids as practiced in the authors’ laboratories.

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Acknowledgments

The various research projects were funded in whole or in part by The Medical Research Council of Canada, Ottawa, Canada, The Heart and Stroke Foundation of Ontario, Toronto, Ontario and The Academy of Finland, Helsinki, Finland.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada

    Arnis Kuksis

  2. Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland

    Jukka-Pekka Suomela, Marko Tarvainen & Heikki Kallio

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  1. Arnis Kuksis
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  2. Jukka-Pekka Suomela
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  3. Marko Tarvainen
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    Donald Armstrong

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Kuksis, A., Suomela, JP., Tarvainen, M., Kallio, H. (2009). Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography, HPLC, and On-line MS. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology™, vol 580. Humana Press. https://doi.org/10.1007/978-1-60761-325-1_4

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