Summary
Applications of tandem mass spectrometry in the field of lipid clinical chemistry are considered. Haematological and biochemical advantages are presented favoring the choice of red blood cell membranes as a starting material in a wide variety of biomedical fields. Practical considerations are discussed with respect to methods of sampling, storage, and lipid extraction of red blood cells. The chapter describes the capabilities of a direct infusion of raw lipid extracts in the electro-spray ionization source compared with the more sophisticated method of high-performance liquid chromatography coupled with hybrid tandem mass spectrometry. Both methods have been evaluated and have been shown to be suitable for diagnosis and/or monitoring for a variety of human disorders.
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This work was aided by a grant from the Human Frontier Science Programme (RGP0016/2005C).
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Quinn, P.J., Rainteau, D., Wolf, C. (2009). Lipidomics of the Red Cell in Diagnosis of Human Disorders. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology, vol 579. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-322-0_7
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DOI: https://doi.org/10.1007/978-1-60761-322-0_7
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