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Combining Lipidomics and Proteomics of Human Cerebrospinal Fluids

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 579))

Summary

Lipids from dietary sources or from de novo synthesis are transported while bound to proteins to other tissues where they are used for cell membrane synthesis or stored for energy generation. In cell membranes or in plasma, lipids can undergo several modifications that are important in cell function. Several proteins orchestrate the transport, biosynthesis, and modification of lipids. Thus, the intersection of lipids and proteins is important in human metabolic pathways. Recent advances in mass spectrometry and bioinformatics have made it possible to obtain compositional (structural and functional) data of lipid molecular species and proteins in biological samples. This combination of lipidomics and proteomics is advantageous because it allows us to better define biochemical pathways, discover new drug targets, and better understand the pathophysiology of several diseases.

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Acknowlegments

We thank Susan Onami and Elena Oborina for technical help. We appreciate the support and critical discussion with Drs. Michael Harrington, Andreas Hulmer, and Roger Biringer. This work was supported in part by NIH grants RO1# NS43295; institutional support (HMRI), the Norris, Jamison and Glide Foundations and donations from the Dunlevey, Hezlep and Posthuma families.

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Authors and Affiliations

  1. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, USA

    Alfred N. Fonteh

  2. Scripps College, Claremont, CA, USA

    Rachel D. Fisher

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  1. Alfred N. Fonteh
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  2. Rachel D. Fisher
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  1. Golden Leaf Rd. 215, Mars Hill, 28754, U.S.A.

    Donald Armstrong

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Fonteh, A.N., Fisher, R.D. (2009). Combining Lipidomics and Proteomics of Human Cerebrospinal Fluids. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology, vol 579. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-322-0_4

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  • DOI: https://doi.org/10.1007/978-1-60761-322-0_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-321-3

  • Online ISBN: 978-1-60761-322-0

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