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Analysis of Lipid Particles from Yeast

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 579))

Summary

Quantitative analysis of components from different subcellular fractions is a key to the understanding of metabolic function as well as to the origin, the biogenesis, and the crosstalk of organelles. The yeast is an excellent model organism to address such questions from the biochemical, molecular biological, and cell biological viewpoints. A yeast organelle which gained much interest during the last decade is the lipid particle/droplet (LP), a storage compartment for nonpolar lipids but at the same time an organelle actively contributing to cellular metabolism. In this chapter, we describe methods and techniques that are commonly used to analyze lipids from LP at the molecular level by thin-layer chromatography, gas–liquid chromatography, and mass spectrometry. We provide an easy to follow guideline for the isolation of these organelles, the qualitative and quantitative analysis of lipid components and show results obtained with these methods.

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Abbreviations

CWW:

Cell wet weight

ER:

Endoplasmic reticulum

GLC:

Gas–liquid chromatography

LP:

Lipid particle(s)/droplet(s)

MS:

Mass spectrometry

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PI:

Phosphatidylinositol

PS:

Phosphatidylserine

RT:

Room temperature

STE:

Steryl ester

TAG:

Triacylglycerol

TLC:

Thin-layer chromatography

References

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Acknowledgments

Work on yeast nonpolar lipid metabolism in our laboratory was recently supported by the Fonds zur Förderung der wissenschaftlichen Forschung in Österreich (projects 15141, 18857 and W901-B05 to G.D.).

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Authors and Affiliations

  1. Institute of Biochemistry, Graz University of Technology, Graz, Austria

    Melanie Connerth, Karlheinz Grillitsch, Harald Köfeler & Günther Daum

Authors
  1. Melanie Connerth
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  2. Karlheinz Grillitsch
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  3. Harald Köfeler
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  4. Günther Daum
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Editor information

Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, U.S.A.

    Donald Armstrong

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Connerth, M., Grillitsch, K., Köfeler, H., Daum, G. (2009). Analysis of Lipid Particles from Yeast. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology, vol 579. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-322-0_18

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  • DOI: https://doi.org/10.1007/978-1-60761-322-0_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-321-3

  • Online ISBN: 978-1-60761-322-0

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