Summary
A fully automated two-dimensional electrophoresis (2DE) system for rapid and reproducible protein analysis is described. 2DE that is a combination of isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein expression analysis. Here, all the operations are achieved in a shorter time and all the transferring procedures are performed automatically. The system completed the entire process within 1.5 h. A device configuration, operational procedure, and data analysis are described using this system.
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Acknowledgments
The authors would like to thank our project members from National Institute of Advanced Industrial Science and Technology (AIST), Toppan Printing Co. Ltd, Sharp Corporation, Astellas Pharma Inc., Tokyo University of Technology and Kumamoto University. This work was financially supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Hiratsuka, A., Yokoyama, K. (2009). Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis. In: Koga, H. (eds) Reverse Chemical Genetics. Methods in Molecular Biology™, vol 577. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-232-2_12
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DOI: https://doi.org/10.1007/978-1-60761-232-2_12
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-60761-232-2
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