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MALDI MS

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Proteomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 564))

Summary

Matrix-assisted laser desorption/ionization (MALDI) is a key technique in mass spectrometry (MS)-based proteomics. MALDI MS is extremely sensitive, easy-to-apply, and relatively tolerant to contaminants. Its high-speed data acquisition and large-scale, off-line sample preparation has made it once again the focus for high-throughput proteomic analyses. These and other unique properties of MALDI offer new possibilities in applications such as rapid molecular profiling and imaging by MS. Proteomics and its employment in Systems Biology and other areas that require sensitive and high-throughput bioanalytical techniques greatly depend on these methodologies. This chapter provides a basic introduction to the MALDI methodology and its general application in proteomic research. It describes the basic MALDI sample preparation steps and two easy-to-follow examples for protein identification including extensive notes on these topics with practical tips that are often not available in the Subheadings 2 and 3 of research articles.

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Acknowledgments

The author thanks Dr. Soren Naaby-Hansen for providing the 2DE gel sample, Dr. Ali Tiss for acquiring the data of the human serum sample, and Dr. Eckhard Nordhoff for critical reading of the manuscript. This work was partially supported by the MRC through grant G0301107.

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  1. The BioCentre and Department of Chemistry, The University of Reading, Reading, UK

    Rainer Cramer

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  1. Rainer Cramer
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Correspondence to Rainer Cramer .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Cramer, R. (2009). MALDI MS. In: Reinders, J., Sickmann, A. (eds) Proteomics. Methods in Molecular Biology™, vol 564. Humana Press. https://doi.org/10.1007/978-1-60761-157-8_5

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    • DOI: https://doi.org/10.1007/978-1-60761-157-8_5

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-60761-156-1

    • Online ISBN: 978-1-60761-157-8

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