Summary
Electrospray ionization mass spectrometry (ESI-MS) is an efficient soft ionization procedure for macro biomolecules. However, it is a rather delicate process to produce charged molecules for mass-to-charge ratio (m/z) based measurement. In this chapter, the mechanism of ESI is briefly presented, and the experimental pipeline for quantitative profiling of plasma proteins (prefractionation immunodepletion, protein isotope tagging, 2D-HPLC separation of intact proteins, and LC-MS) is presented as applied by our group in studies of cancer biomarker discovery.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Wang, H., Hanash, S. (2009). Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis. In: Reinders, J., Sickmann, A. (eds) Proteomics. Methods in Molecular Biology™, vol 564. Humana Press. https://doi.org/10.1007/978-1-60761-157-8_13
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DOI: https://doi.org/10.1007/978-1-60761-157-8_13
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