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Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis

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Protocols for Adult Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 621))

Abstract

Endothelial progenitor cell-based therapy offers great potential to reduce morbidity and mortality in patients with vascular diseases. In animal models of ischemia, circulating endothelial progenitor cells (EPCs) have been shown to home to sites of active angiogenesis and differentiate into endothelial cells in response to tissue ischemia, vascular trauma, or tumor growth. These studies indicate a thorough understanding of EPCs function and role in angiogenesis as a potential therapeutic target for vascular diseases. In this chapter, we describe the procedures for: (1) isolation of EPCs from circulating blood and bone marrow; (2) culturing these cells; (3) characterization of EPCs by FCAS, DiI-ac-LDL up-taking, and FITC-UEA-1 binding assays; and (4) determination of EPCs’ function by examining proliferation, migration, and tube formation in vitro.

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Author information

Authors and Affiliations

  1. Department of Anesthesia and Perioperative Care, Center for Cerebrovascular Research, University of California, San Francisco, CA, USA

    Steve Khachi & Yiqian Zhu

  2. Department of Anesthesia and Perioperative Care and Department of Neurological Surgery, Center for Cerebrovascular Research, University of California, San Francisco, CA, USA

    Guo-Yuan Yang

Authors
  1. Steve Khachi
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  2. Yiqian Zhu
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  3. Guo-Yuan Yang
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Corresponding author

Correspondence to Guo-Yuan Yang .

Editor information

Editors and Affiliations

  1. Dept. Bioengineering, University of California, Berkeley, Evans Hall 479, Berkeley, 94720-1762, USA

    Irina M. Conboy

  2. Helen Wills Neuroscience Inst., University of California, Berkeley, Stanley Hall 176, Berkeley, 94720-3220, USA

    David V. Schaffer

  3. Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Cyclotron Rd. 1, Berkeley, 94720, USA

    Mary Helen Barcellos-Hoff

  4. Dept. Bioengineering, University of California, Berkeley, Stanley Hall, Berkeley, 94720-1762, USA

    Song Li

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Khachi, S., Zhu, Y., Yang, GY. (2010). Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_9

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  • DOI: https://doi.org/10.1007/978-1-60761-063-2_9

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-062-5

  • Online ISBN: 978-1-60761-063-2

  • eBook Packages: Springer Protocols

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