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Protocols for Adult Stem Cells pp 89–102Cite as

In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 621))

Abstract

A neural degenerative disease is characterized by the deterioration of neural tissue and subsequent loss of function. The in vivo engraftment of neural stem cells is a promising approach to the functional replacement of neural tissue with the ultimate goal of regaining lost function. In addition, by studying the behavior of engrafted neural stem cells in healthy and diseased tissue, insight can be gained into the extracellular and intracellular mechanisms which regulate stem cell behavior in vivo. Adult hippocampal neural progenitor cells (AHNPCs) are one potential source of cells that can be used to this goal. In this chapter, we describe some of the in vivo techniques necessary to study hippocampal progenitors in the adult rat, including engraftment and analysis by immunofluorescent staining. These techniques are important for studying AHNPCs within the physiologically relevant environment of the adult brain rather than in a culture dish.

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References

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Acknowledgments

We acknowledge Jae Jang and Brandi Ormerod for helpful technical comments. This work was funded by NIH T32 GM007352 (to MJR), an NSF Graduate Research Fellowship (to JP), a California Institute for Regenerative Medicine Training Grant (T1-00007), and NSF BES-0629202.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of California, Berkeley, CA, USA

    Matthew J. Robertson, Joseph Peltier & David V. Schaffer

Authors
  1. Matthew J. Robertson
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  2. Joseph Peltier
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  3. David V. Schaffer
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Corresponding author

Correspondence to David V. Schaffer .

Editor information

Editors and Affiliations

  1. Dept. Bioengineering, University of California, Berkeley, Evans Hall 479, Berkeley, 94720-1762, USA

    Irina M. Conboy

  2. Helen Wills Neuroscience Inst., University of California, Berkeley, Stanley Hall 176, Berkeley, 94720-3220, USA

    David V. Schaffer

  3. Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Cyclotron Rd. 1, Berkeley, 94720, USA

    Mary Helen Barcellos-Hoff

  4. Dept. Bioengineering, University of California, Berkeley, Stanley Hall, Berkeley, 94720-1762, USA

    Song Li

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Robertson, M.J., Peltier, J., Schaffer, D.V. (2010). In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_6

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  • DOI: https://doi.org/10.1007/978-1-60761-063-2_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-062-5

  • Online ISBN: 978-1-60761-063-2

  • eBook Packages: Springer Protocols

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