Abstract
Adult neurogenesis, or the creation of new neurons in adult organisms, is an exciting recent area of neurological research. The subgranular zone of the adult hippocampus is one area where hippocampal neural progenitors generate new neurons that functionally integrate into existing neuronal circuitry. Given the role that the hippocampus plays in learning and memory consolidation and its vulnerability to neurological diseases and conditions, such as Alzheimer’s disease, understanding the mechanisms controlling the self-renewal and differentiation of neural progenitor cells is a critical first step in developing novel disease treatments. In this and subsequent chapters, we describe many of the in vivo and in vitro techniques necessary to study hippocampal progenitors in the adult rat. Specifically, this chapter details isolation of progenitors from the adult rat for the establishment of in vitro culture.
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Acknowledgments
We acknowledge Theo Palmer for helpful technical comments and Matthew Robertson for critical reading of the manuscript. This work was funded by an NSF Graduate Research Fellowship (to JP), a California Institute for Regenerative Medicine Training Grant (T1-00007), and NSF BES-0629202.
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Peltier, J., Ormerod, B.K., Schaffer, D.V. (2010). Isolation of Adult Hippocampal Neural Progenitors. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_4
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DOI: https://doi.org/10.1007/978-1-60761-063-2_4
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