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Protocols for Adult Stem Cells pp 49–55Cite as

Use of Stem Cell Markers in Dissociated Mammary Populations

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 621))

Abstract

The regenerative potential of mammary epithelium facilitates assessment of the “stemness” of any epithelial subpopulation in transplantation assays. Thus, mammary tissue can be dissociated into single cells, stained for cell surface markers of interest and classified using fluorescence-activated cell sorting. The selected cells can then be transplanted into epithelium-devoided fat pads from recipient hosts. Recent publications have described markers that enrich for mammary repopulating potential. Here, we describe the materials and methods necessary to sort cells according to these markers. This approach can be used interchangeably with other cell surface markers with slight variation to the protocol.

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Acknowledgments

This work was supported by a predoctoral fellowship to RFG from the Department of Defense Breast Cancer Research Program (DAMD 17-03-1-0594), grants from the same institution to COS (DAMD 17-00-1-0227 and DAMD 17-00-1-0306), a grant to BEW from the National Cancer Institute (CA 8424306), and a grant to MHBH funded by the National Institute of Environmental Health Sciences and the National Cancer Institute (U01ES012801).

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Authors and Affiliations

  1. Department of Surgery, University of Utah, Huntsman Cancer Institute, Salt Lake City, UT, 84112, USA

    Dawne N. Shelton & Bryan E. Welm

  2. Development Biology Program, Sloan-Kettering, Institute, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA

    Rodrigo Fernandez-Gonzalez

  3. Langone School of Medicine, New York University, New York, NY, 10016, USA

    Irineu Illa-Bochaca & Mary Helen Barcellos-Hoff

  4. Morphology and Imaging Group and Cancer Imaging Laboratory, Center for Applied Medical Research, University of Navarre, Pamplona, 31008, Navarre, Spain

    Carlos Ortiz-de-Solorzano

Authors
  1. Dawne N. Shelton
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  2. Rodrigo Fernandez-Gonzalez
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  3. Irineu Illa-Bochaca
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  4. Carlos Ortiz-de-Solorzano
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  5. Mary Helen Barcellos-Hoff
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  6. Bryan E. Welm
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Corresponding author

Correspondence to Mary Helen Barcellos-Hoff .

Editor information

Editors and Affiliations

  1. Dept. Bioengineering, University of California, Berkeley, Evans Hall 479, Berkeley, 94720-1762, USA

    Irina M. Conboy

  2. Helen Wills Neuroscience Inst., University of California, Berkeley, Stanley Hall 176, Berkeley, 94720-3220, USA

    David V. Schaffer

  3. Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Cyclotron Rd. 1, Berkeley, 94720, USA

    Mary Helen Barcellos-Hoff

  4. Dept. Bioengineering, University of California, Berkeley, Stanley Hall, Berkeley, 94720-1762, USA

    Song Li

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© 2010 Springer Science+Business Media, LLC

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Shelton, D.N., Fernandez-Gonzalez, R., Illa-Bochaca, I., Ortiz-de-Solorzano, C., Barcellos-Hoff, M.H., Welm, B.E. (2010). Use of Stem Cell Markers in Dissociated Mammary Populations. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_3

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  • DOI: https://doi.org/10.1007/978-1-60761-063-2_3

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-062-5

  • Online ISBN: 978-1-60761-063-2

  • eBook Packages: Springer Protocols

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