Summary
Understanding the dynamics of developmental and cellular processes requires documentation of their changes with appropriate temporal and spatial resolution. Furthermore, simultaneous recording from a population of embryos under identical conditions allows statistical estimates of precision and variability to be made. This chapter describes a protocol for time-lapse microscopy of multiple embryos in parallel developing under tightly controlled conditions. This method is currently best suited to follow tissue-scale morphogenetic movements with temporal resolution in the minute range, for hours or even days. Applications of the method include the comparison of the dynamics of a process of interest between groups of wild-type embryos and their mutant siblings or between embryos treated with different chemical compounds. Temperature control allows for the investigation of the temperature dependence of a process of interest.
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Acknowledgments
We thank Albert Cardona, Gary J. Brouhard, Benjamin Feldman and the workshop of the MPI-CBG for their help in establishing important aspects of the technique; Justin Costa, Sean Megason, and Britta Schroth-Diez for supplying reagents or technical suggestions; and the MPI-CBG fish facility and the members of the Oates lab for support and discussion.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Herrgen, L., Schröter, C., Bajard, L., Oates, A.C. (2009). Multiple Embryo Time-Lapse Imaging of Zebrafish Development. In: Lieschke, G., Oates, A., Kawakami, K. (eds) Zebrafish. Methods in Molecular Biology, vol 546. Humana Press. https://doi.org/10.1007/978-1-60327-977-2_15
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DOI: https://doi.org/10.1007/978-1-60327-977-2_15
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Online ISBN: 978-1-60327-977-2
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