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Discovery of Antibody Biomarkers Using Protein Microarrays of Tumor Antigens Cloned in High Throughput

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Book cover Tumor Biomarker Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 520))

Summary

Development of humoral and cellular immunity against self-cellular proteins in cancer patients is a phenomenal observation. The ability of immune system to sense the presence of the disease and to fight of the disease by generating autoantibodies against tumor antigens makes it a natural biosensor. Several screening technologies have been employed for the identification of tumor-specific antibodies in cancer patients. We have developed a multidimensional approach for the identification of diagnostic antigens that utilizes a combination of high-throughput antigen cloning and protein microarray-based serological detection of complex panels of antigens by exploiting the serum autoantibody repertoire directed toward tumor-associated antigens in cancer patients. Furthermore, validation of these antigens by different bioinformatics and biological approaches will reveal the diagnostic/prognostic utility of these antigens for personalized immunotherapy.

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Acknowledgments

This work was supported by The Barbara and Fred Erb Endowed Chair in Cancer Genetics, The Gail Purtan Ovarian Cancer Research Fund, The Sinai Guild, and grants from The Michigan Life Science Corridor Fund 085P300470, NIH grant CA100740, NIH grant U01 CA117478-01, and The Susan G. Komen Breast Cancer Foundation grant BCTR0504211 (M.A.Tainsky).

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, MI, USA

    Madhumita Chatterjee, Jerzy Wojciechowski & Michael A. Tainsky

Authors
  1. Madhumita Chatterjee
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  2. Jerzy Wojciechowski
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  3. Michael A. Tainsky
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Editors and Affiliations

  1. School of Medicine , Wayne State University, E. Warren Ave. 110, Detroit, 48201, U.S.A.

    Michael A. Tainsky

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Chatterjee, M., Wojciechowski, J., Tainsky, M.A. (2009). Discovery of Antibody Biomarkers Using Protein Microarrays of Tumor Antigens Cloned in High Throughput. In: Tainsky, M. (eds) Tumor Biomarker Discovery. Methods in Molecular Biology, vol 520. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-811-9_3

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  • DOI: https://doi.org/10.1007/978-1-60327-811-9_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-810-2

  • Online ISBN: 978-1-60327-811-9

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