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Tumor Biomarker Discovery pp 315–327Cite as

Magnetic Resonance Spectroscopy of Living Tissues

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 520))

Summary

The comprehensive work of both clinical and basic science colleagues has demonstrated a clear proof of concept for “in vitro discovered- in vivo validated” biomarkers in translational metabolic profiling research using magnetic resonance techniques. Major tissue metabolites (initially discovered by high-resolution in vitro techniques on cancer specimens) can be translated into in vivo protocols based on noninvasive magnetic resonance spectroscopy (MRS). Using 1H- and 31P-MRS on living animals or patients, a decrease in citrate and polyamines in prostate cancer, an increase of cholines in breast cancer, as well as a decreased NAA and an increased lactate in gliomas during cancer progression can be assessed noninvasively. MRS can be used to follow up conventional cytotoxic as well as targeted anticancer therapies, which has been extensively done in animal models of cancer. This review focuses on applications and protocol development for in vivo 1H- and 31P-MRS on small animal models as well as on larger animals in cancer research, diagnosis, and treatment.

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Acknowledgments

This work was supported by the grants from the National Institutes of Health, S10 RR019316 and P30 CA046934.

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Authors and Affiliations

  1. Department of Anesthesiology and Radiology, University of Colorado at Denver and Health Sciences Center, Aurora, CO, USA

    Natalie J. Serkova

  2. Department of Anesthesiology, University of Colorado at Denver and Health Sciences Center, Aurora, CO, USA

    Kendra M. Hasebroock

  3. Department of Radiological Sciences, Colorado State University, Fort Collins, CO, USA

    Susan L. Kraft

Authors
  1. Natalie J. Serkova
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  2. Kendra M. Hasebroock
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  3. Susan L. Kraft
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Corresponding author

Correspondence to Susan L. Kraft .

Editor information

Editors and Affiliations

  1. School of Medicine , Wayne State University, E. Warren Ave. 110, Detroit, 48201, U.S.A.

    Michael A. Tainsky

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Serkova, N.J., Hasebroock, K., Kraft, S. (2009). Magnetic Resonance Spectroscopy of Living Tissues. In: Tainsky, M. (eds) Tumor Biomarker Discovery. Methods in Molecular Biology, vol 520. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-811-9_22

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  • DOI: https://doi.org/10.1007/978-1-60327-811-9_22

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-810-2

  • Online ISBN: 978-1-60327-811-9

  • eBook Packages: Springer Protocols

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