Summary
In this chapter, method of fabricating a cantilever biosensors and their use in measuring the presence of a protein is described. There are many variations in construction of a cantilever sensor. A simple and an easy version is described in this chapter. The specificity of the sensor is obtained by immobilizing an antibody specific to the antigen of interest. The piezoelectric cantilever sensors are very sensitive and can easily detect a 60 kDa protein at 100 fg/mL concentration. Experimental procedure for carrying out detection of a target analyte is outlined and a sample set of results is included.
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Acknowledgments
This work was partially supported by the grants USDA 2006-51110-03641 and EPA R-833007. The sample experiment reported was done by David Maraldo as part of his Ph.D. work.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC
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Mutharasan, R. (2009). Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 504. Humana Press. https://doi.org/10.1007/978-1-60327-569-9_5
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DOI: https://doi.org/10.1007/978-1-60327-569-9_5
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