Summary
Several methods are described in which a biological recognition molecule — a critical element in any biosensor — is immobilized onto a silica or silica-based sensing substrate. Although several variations are described, the methods for covalent immobilization share a common theme and are generally composed of three steps: modification of the surface to add specific functional groups (using appropriate silanes or an amine or carboxyl-containing hydrogel), covalent attachment of a crosslinker through one of its reactive moieties, and finally, covalent linking of the biomolecule (recognition element) to the remaining reactive moiety of the crosslinker. One final method is presented in which the surface is modified with a highly hydrophobic silane and a glycolipid recognition element immobilized, essentially irreversibly, by hydrophobic interactions. All of the methods described have been successfully used to immobilize biological recognition molecules onto sensing surfaces, with full functionality in biosensor-binding assays.
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Bhatia, S. K., Cooney, M. J., Shriver-Lake, L. C., Fare, T. L., and Ligler, F. S. (1991) Immobilization of acetylcholinesterase on solid-surfaces — chemistry and activity studies. Sens. Actuator. B-Chem. 3, 311–317
Duveneck, G. L., Neuschafer, D., and Ehrat, M. (1995) Process for detecting evanescently excited luminescence., Vol. International Patent Go1N 21/77, 21/64
Duveneck, G. L., Pawlak, M., Neuschafer, D., Bar, E., Budach, W., Pieles, U., and Ehrat, M. (1997) Novel bioaffinity sensors for trace analysis based on luminescence excitation by planar waveguides. Sens. Actuator. B-Chem. 38/39, 88–95
Liron, Z., Tender, L. M., Golden, J. P., and Ligler, F. S. (2002) Voltage-induced inhibition of antigen-antibody binding at conducting optical waveguides. Biosens. Bioelectron. 17, 489–494
Pawlak, M., Grell, E., Schick, E., Anselmettin, D., and Ehrat, M. (1998) Functional immobilization of biomembrane fragments on planar waveguides for the investigation of site-directed ligand binding by surface confined fluorescence. Faraday Discus. 111, 273–288
Sapsford, K. E., Rowe Taitt, C. A., and Ligler, F. S. (2002) Planar waveguides for fluorescence biosensors. In: Optical biosensors: Present and future (Ligler, F. S. & Rowe Taitt, C. A., eds.). Elsevier, The Netherlands, pp. 95–122
Bernard, A., Michel, B., and Delamarche, E. (2001) Micromosaic immunoassays. Anal. Chem. 73, 8–12
Delamarche, E., Bernard, A., Michel, B., and Biebuyek, H. (1997) Patterned delivery of immunoglobulins to surfaces using microflu-idic networks. Science 276, 779–781
Gauglitz, G. (2005) Direct optical sensors: principles and selected applications. Anal. Bioanal. Chem. 381, 141–155
Golden, J., Shriver-Lake, L., Sapsford, K., and Ligler, F. (2005) A “Do-it-yourself” array biosensor. Methods 37, 65–72
Ngundi, M. M., Taitt, C. R., McMurry, S. A., Kahne, D., and Ligler, F. S. (2006) Detection of bacterial toxins with mon-osaccharide arrays. Biosens. Bioelectron. 21, 1195–1201
Rowe, C. A., Scruggs, S. B., Feldstein, M. J., Golden, J. P., and Ligler, F. S. (1999) An array immunosensor for simultaneous detection of clinical analytes. Anal. Chem. 71, 433–439
Sapsford, K. E., Rasooly, A., Taitt, C. R., and Ligler, F. S. (2004) Rapid detection of Campylobacter and Shigella species in food samples using an array biosensor. Anal. Chem. 76, 433–440
Sapsford, K. E., Taitt, C. R., Loo, N., and Ligler, F. S. (2005) Biosensor detection of botulinum toxoid A and staphylococcal enterotoxin B in food. Appl. Environ. Micro-biol. 71, 5590–5592
Taitt, C. R., Anderson, G. P. , Lingerfelt, B. M., Feldstein, M. J., and Ligler, F. S. (2002) Nine-analyte detection using an array-based biosensor. Anal. Chem. 74, 6114–6120
Tschmelak, J., Kumpf, M., Proll, G., and Gauglitz, G. (2004) Biosensor for seven sul-phonamides in drinking, ground, and surface water with difficult matrices. Anal. Lett. 37, 1701–1718
Tschmelak, J., Proll, G., and Gauglitz, G. (2004) Verification of performance with the automated direct optical TIRF immunosen-sor (River Analyser) in single and multi-ana-lyte assays with real water samples. Biosens. Bioelectron. 20, 743–752
Moreno-Bondi, M. C., Taitt, C. R., Shriver-Lake, L. C., and Ligler, F. S. (2006) Multiplexed measurement of serum antibodies using an array biosensor. Biosens. Bioelectron. 21, 1880–1886
Ngundi, M. M., Shriver-Lake, L. C., Moore, M. H., Ligler, F. S., and Taitt, C. R. (2006) Multiplexed detection of mycotoxins in foods with a regenerable array. J. Food Prot. 69, 3047–3051
Martin, B. D., Lindhardt, R. J., and Dordick, J. S. (1998) Highly swelling hydrogels from ordered galactose-based polyacrylates. Bio-materials 19, 69–76
Cras, J. J., Rowe-Taitt, C. A., Nivens, D. A., and Ligler, F. S. (1999) Comparison of chemical cleaning methods of glass in preparation for silanization. Biosens. Bioelectron. 14, 683–688
Shriver-Lake, L. C. (1998) Silane-modified surfaces for biomaterial immobilization. In: Immobilized biomolecules in analysis: A practical approach (Cass, T. & Ligler, F. S., eds.). Oxford University Press, Oxford
Golden, J. P. , Taitt, C. R., Shriver-Lake, L. C., Shubin, Y. S., and Ligler, F. S. (2005) A portable automated multianalyte biosensor. Talanta 65, 1078–1085
Charles, P. T., Goldman, E. R., Rangasammy, J. G., Schauer, C. L., Chen, M.-S., and Taitt, C. R. (2004) Fabrication and characterization of 3D hydrogel microarrays to measure anti-genicity and antibody functionality for biosensor applications. Biosens. Bioelectron. 20, 753–764
Ngundi, M. M., Kulagina, N. V., Anderson, G. P., and Taitt, C. R. (2006) Nonantibody-based recognition: alternative molecules for detection of pathogens. Exp. Rev. Proteomics 3, 511–524
Rowe-Taitt, C. A., Cras, J. J., Patterson, C. H., Golden, J. P., and Ligler, F. S. (2000) A ganglioside-based assay for cholera toxin using an array biosensor. Anal. Biochem. 281, 123–133
Acknowledgments
The development of these methods was in part funded by the Office of Naval Research. The views expressed here are those of the authors and do not represent those of the U.S. Navy, the U.S. Department of Defense or the U.S. Government.
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Shriver-Lake, L.C., Charles, P.T., Taitt, C.R. (2009). Immobilization of Biomolecules onto Silica and Silica-Based Surfaces for Use in Planar Array Biosensors. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 504. Humana Press. https://doi.org/10.1007/978-1-60327-569-9_23
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DOI: https://doi.org/10.1007/978-1-60327-569-9_23
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