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Biosensors and Biodetection pp 3–22Cite as

A Set of Piezoelectric Biosensors Using Cholinesterases

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 504))

Summary

Piezoelectric sensors have become a versatile tool in biosensorics to study protein—protein and protein—small molecule interactions. Here we present theoretical background on piezoelectric sensors and instructions, how to modify their surface with various recognition elements for cholinesterases. These recognition elements comprise an organophosphate (paraoxon), a cocaine derivative (BZE-DADOO), and a tricyclic, aromatic compound (propidium). Additionally, a guide to the kinetic evaluation of the obtained binding curves is given in this chapter.

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Acknowledgments

The authors thank the European Union (Marie-Curie-fellowship HPMD-CT-2001-00062) and the “International Max Planck Research School on Biomimetic Systems” (PhD scholarship) for financial support.

Author information

Authors and Affiliations

  1. Department of Analytical Biochemistry, University of Potsdam, Potsdam, Germany

    Carsten Teller, Alexander Makower & Frieder W. Scheller

  2. Department of Analytical Biochemistry, University of Potsdam, Potsdam, Germany Biophysical Engineering Group, University of Twente, Enschede, The Netherlands

    Jan Halámek

Authors
  1. Carsten Teller
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  2. Jan Halámek
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  3. Alexander Makower
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  4. Frieder W. Scheller
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Editor information

Editors and Affiliations

  1. FDA Center for Devices and Radiological Health, Silver Spring, MD, USA

    Avraham Rasooly

  2. National Cancer Institute, Bethesda, MD, USA

    Avraham Rasooly

  3. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA

    Keith E. Herold

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC

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Teller, C., Halámek, J., Makower, A., Scheller, F.W. (2009). A Set of Piezoelectric Biosensors Using Cholinesterases. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 504. Humana Press. https://doi.org/10.1007/978-1-60327-569-9_1

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  • DOI: https://doi.org/10.1007/978-1-60327-569-9_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-568-2

  • Online ISBN: 978-1-60327-569-9

  • eBook Packages: Springer Protocols

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