Summary
Spectral SPR biosensor is a useful system for a rapid analysis of protein arrays, as the biosensor with a fiber optic spectrometer can be easily aligned with the reflected light from protein arrays. The spectral SPR biosensor was constructed by Kretschmann geometry, based on the wavelength interrogation with various modules such as protein arrays, optical unit, programs for data acquisition and processing, and a motorized x–y stage for scanning. Protein arrays consist of glass/gold film/linker layer/protein/air. The surface of gold arrays was modified with poly(diallyldimethylammonium chloride) and 11-mercap-toundecanoic acid to immobilize mumps virus. The virus arrays were used to analyze anti-mumps virus in a buffer or human serum by the line-scanning mode of the spectral SPR biosensor. The array-based spectral SPR biosensor has a strong potential for a high-throughput serodiagnosis of infectious diseases.
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Acknowledgments
This work was supported in part by a grant of the Korea Health 21 R&D Project, Ministry of Health, Welfare & Family Affairs, Republic of Korea (A030003).
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC
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Yuk, J.S., Ha, KS. (2009). Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 503. Humana Press. https://doi.org/10.1007/978-1-60327-567-5_3
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DOI: https://doi.org/10.1007/978-1-60327-567-5_3
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