Skip to main content

DNA Analysis with a Photo-Diode Array Sensor

  • Protocol

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 503))

A simple instrument or device for easy DNA analysis is required. A combination of bioluminometric assay and photo-detection with an inexpensive photo-diode array provides a simple instrument for various DNA analyses. Its characteristics and applications for DNA analyses are described.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Kheterpal, I. and Mathies, R.A. (1999) Capillary array electrophoresis DNA sequencing. Anal Chem, 71, 31A–37A

    CAS  PubMed  Google Scholar 

  2. Zubritsky, E. (2002) How analytical chemists saved the human genome project. Anal Chem, 74, 23A–26A

    PubMed  Google Scholar 

  3. Kambara, H. and Takahashi, S. (1993) Mul-tiple-sheathflow capillary array DNA analyser. Nature, 361, 565–566

    Article  CAS  PubMed  Google Scholar 

  4. Ronaghi, M., Uhlen, M. and Nyren, P. (1998) A sequencing method based on real-time pyro-phosphate. Science, 281, 363–365

    Article  CAS  PubMed  Google Scholar 

  5. Zhou, G.-H., Kamahori, M., Okano, K., Harada, K. and Kambara, H. (2001) Miniaturized pyrose-quencer for DNA analysis with capillaries to deliver deoxynucleotides. Electrophoresis, 22, 3497–3504

    Article  CAS  PubMed  Google Scholar 

  6. Zhou, G.-H., Kajiyama, T., Gotou, M., Kishi-moto, A., Suzuki, S. and Kambara, H. (2006) Enzyme system for improving the detection limit in pyrosequencing. Anal Chem, 78, 4482–4489

    Article  CAS  PubMed  Google Scholar 

  7. Zhou, G.-H., Gotou, M., Kajiyama, T. and Kambara, H. (2005) Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation (BATI). Nucleic Acids Res, 33, e133

    Article  PubMed  Google Scholar 

  8. Gharizadeh, B., Nordstrom, T., Ahma-dian, A., Ronaghi, M. and Nyren, P. (2002) Long-read pyrosequencing using pure 2′-deoxyadenosine-5′-O'- (1-thiotri-phosphate) Sp-isomer. Anal Biochem, 301, 82–90

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The author would like to thank Dr Tomoharu Kajiyama and Dr Akihiko Kishimoto for the development of technologies and instruments, and Ms Sumiyo Takiguchi and Ms Mari Goto for their help in experiments.

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Kambara, H., Zhou, G. (2009). DNA Analysis with a Photo-Diode Array Sensor. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 503. Humana Press. https://doi.org/10.1007/978-1-60327-567-5_19

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-567-5_19

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-566-8

  • Online ISBN: 978-1-60327-567-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics