Abstract
Plants communicate with their surrounding ecosystems using a diverse array of volatile metabolites that are indicative of the physiological status of the emitter. A variety of systems have been adapted to capture, analyze, identify, and quantify airborne metabolites released by plants. Metabolomic experiments typically involve four steps: sample collection, preparation, product separation, and data analysis. To date, two different types of headspace sampling, static and dynamic, are widely used for volatile metabolome investigation. For static headspace analysis, solid-phase microextraction (SPME) is used to sample volatiles while push and pull as well as closed-loop stripping methods are used for dynamic headspace sampling. After collection, volatile blends are most efficiently and routinely separated prior to analysis using gas chromatography (GC). Sample preparation is simplified because derivatization is not needed with volatile metabolites. GC coupled to detection by electron impact mass spectrometry (EI-MS) provides high chromatographic resolution, sensitivity, compound-specific detection, quantitation, and the potential to identify unknowns by characteristic and reproducible fragmentation spectra in addition to retention time. A variety of resources can be used to identify unknown compounds in a given volatile sample including >600,000 compounds with known mass spectra catalogued in searchable mass spectral libraries.
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Pichersky, E., Noel, J.P., and Dudareva, N. (2006) Biosynthesis of plant volatiles: nature's diversity and ingenuity. Science. 311, 808–811.
Dudareva, N., Negre, F., Nagegowda, D.A., and Orlova, I. (2006) Plant volatiles: recent advances and future perspectives. Crit. Rev. Plant Sci. 25, 417–440.
Knudsen, J.T., Eriksson, R., Gershenzon, J., and Stahl, B. (2006) Diversity and distribution of floral scent. Bot. Rev. 72, 1–120.
Acree, T. and Arn, H. (2004) Flavornet and human odor space. http://www.flavornet.org/index.html. Accessed January 10, 2008.
Tholl, D., Boland, W., Hansel, A., Loreto, F., Rose, U.S.R., and Schnitzler, J.P. (2006) Practical approaches to plant volatile analysis. Plant J. 45, 540–560.
Tholl, D. and Rose, U.S.R. (2006) Detection and identification of floral scent compounds. In: Dudareva, N., Pichersky, E., editors. Biology of Floral Scent. Boca Raton, FL, USA: CRC/Taylor & Francis;. pp. 3–25.
Arthur, C. and Pawliszyn, J. (1990) Solid phase microextraction with thermal desorption using fused silica optical fibers. Anal. Chem. 62, 2145–2148.
Bicchi, C., Drigo, S., and Rubiolo, P. (2000) Influence of fibre coating in headspace solid-phase microextraction–gas chromatographic analysis of aromatic and medicinal plants. J. Chromatogr. A. 892, 469–485.
Górecki, T., Yu, X., and Pawliszyn, J. (1999) Theory of analyte extraction by selected porous polymer SPME fibres. Analyst. 124, 643–649.
Barták, P., Bednár, P., Cáp, L., Ondráková, L., and Stránsky, Z. (2003) SPME – A valuable tool for investigation of flower scent. J. Sep. Sci. 26, 715–721.
Aharoni, A., Giri, A.P., Deuerlein, S., Griepink, F., de Kogel ,W.J., Verstappen, F.W.A., Verhoeven, H.A., Jongsma, M.A., Schwab, W., and Bouwmeester, H.J. (2003) Terpenoid metabolism in wild-type and transgenic Arabidopsis plants. Plant Cell. 15, 2866–2884.
Vas, G. and Vékey, K. (2004) Solid-phase microextraction: a powerful sample preparation tool prior to mass spectrometric analysis. J. Mass Spectrom. 39, 233–254.
D'Alessandro, M. and Turlings, T.C.J. (2006) Advances and challenges in the identification of volatiles that mediate interactions among plants and arthropods. Analyst. 131, 24–32.
Shurig, V. (2001) Separation of enantiomers by gas chromatography. J. Chromatogr. A. 906, 275–299.
Shurig, V. (2002) Chiral separations using gas chromatography. Trends. Anal. Chem. 21, 647–661.
Raguso, R.A. and Pellmyr, O. (1998) Dynamic headspace analysis of floral volatiles: a comparison of methods. Oikos. 81, 238–254.
Acknowledgments
This work was supported by grants from the National Science Foundation (grant no. MCB-0615700), the USDA Cooperative State Research, Education, and Extension Service (grant no. 2005-35318-16207), and the Fred Gloeckner Foundation.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Qualley, A.V., Dudareva, N. (2009). Metabolomics of Plant Volatiles. In: Belostotsky, D. (eds) Plant Systems Biology. Methods in Molecular Biology™, vol 553. Humana Press. https://doi.org/10.1007/978-1-60327-563-7_17
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DOI: https://doi.org/10.1007/978-1-60327-563-7_17
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