Summary
NF-κB plays a pivotal role in immunity and inflammation and is considered to be a promising candidate for drug development. However, global suppression of NF-κB may have undesirable side-effects. Our data and the results of others suggest that each of the five NF-κB subunits may have a specific function in controlling the expression of inflammatory mediators in immune cells. Identifying the role for each NF-κB subunit in primary human immune cells will allow a more targeted approach to inhibiting NF-κB subunit-specific cellular functions. However, results obtained with primary human cells can often be inconsistent due to donor heterogeneity. Therefore one possible approach could be to generate human immune cell lines with stably inhibited expression of specific NF-κB subunit(s) as described in this chapter.
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Acknowledgments
We would like to thank Drs Nicole Horwood and Bernard Gregory (Kennedy Institute) for providing us with the GATEWAY compatible pENTRY-U6 and pLenti_LV5 vectors. This work was supported by the MRC New Investigator Award #75548 to IU.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Goh, F.G., Banks, H., Udalova, I.A. (2009). Detecting and Modulating the NF-kB Activity in Human Immune Cells: Generation of Human Cell Lines with Altered Levels of NF-κB. In: Kozlov, S.V. (eds) Inflammation and Cancer. Methods in Molecular Biology™, vol 512. Humana Press. https://doi.org/10.1007/978-1-60327-530-9_4
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DOI: https://doi.org/10.1007/978-1-60327-530-9_4
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