Application of Phage Display to Discovery of Tumor-Specific Homing Peptides: Developing Strategies for Therapy and Molecular Imaging of Cancer
Selective delivery of drugs or imaging dyes to tumor is the central challenge for improving existing therapy and diagnosis of cancer. Phage display of random peptides has been used to identify homing peptides that are specific to tumor cells or tumor blood vessels. These homing peptides can be conjugated with imaging agents or therapeutic drugs and may be a promising tool for affinity-based targeted delivery of imaging agents and drugs. This chapter describes the procedures for phage display selection of peptides that selectively bind to bladder tumor cells and home to tumor tissues in vivo. It also describes the application of the tumor-specific peptide to the detection of bladder cancer cells in the patient urine.
Key wordsBladder cancer Homing peptide Phage display Molecular imaging Targeted delivery
This work was supported by the Ministry of Science and Technology (F104AA010003-06A0101-00310), grant No. RTI04-01-01 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE), and Brain Korea 21 Project in 2006.
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