Abstract
Acrolein is an α,β-unsaturated aldehyde with enormous capacity of reaction, occurs in the air like a pollutant, but it is (we know now) an important lipid peroxidation product as well. The compound is one of the several aldehydes produced from fatty acid oxidation, although it is particularly important because it constitutes the major electrophyle aldehyde derived from lipid oxidation. Acrolein can be formed actively from oxidized fatty acids and undergo aldolic condensation in alkaline pH; this is a particular characteristic that we have used in its process of separation with capillary electrophoresis. We have shown that the oxidation of unsaturated fatty acids forms acrolein, and that the use of capillary electrophoresis to be a powerful, sensitive, and attractive method for separation, identification, and quantization of this and other aldehydes from in vitro lipid peroxidation.
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References
Uchida, K., Kanematsu, M., Sakai, K., Matsuda, T., Hattori, N., Mizuno, Y., et al. (1999) Protein-bound acrolein: potential markers for oxidative stress. Proc Natl Acad Sci USA 95, 4882–4887.
Luo, J., and Shi, R. (2004) Acrolien induces axolemmal disruption, oxidative stress, and mitochondrial impairment in spinal cord tissue. Neurochem Int 44, 475–486.
Luo, J., and Shi, R. (2004) Acrolien induces oxidative stress in brain mitochondria. Neurochem Int 46, 443–452.
Noiri, E., Yamada, S., Nakao, A., Tsuchiya, M., Masaki, I., Fujing, K., et al. (2002) Serum protein acrolein adducts: utility in detecting oxidant stress in hemodialysis patients and reversal using a vitamin E-bonded hemodialyzer. Free Radic Biol Med 33, 1651–1656.
Arikketh, D., Niranjali, S., and Devaraj, H. (2004) Detection of acrolein-lysine adducts in plasma low-density lipoprotein and in aorta of cyclophosphamide-administered rats. Arch Toxicol 78, 397–401.
Calingasan, N.Y., Uchida, K., and Gibson, G.E. (1999). Protein-bound acrolein: a novel marker of oxidative stress in Alzheimer’s disease. J Neurochem 72, 751–756.
al-Rawithi, S., el-Yazigi, A., and Nicholls, P.J. (1993) Determination of acrolein in urine by liquid chromatography and fluorescence detection of its quinoline derivative. Pharm Res 10, 1587–1590.
Paci, A., Rieutord, A., Guillaume, D., Traoré, F., Ropenga, J., Husson, H.P., et al. (2000) Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin 3. J Chromatogr B Biomed Sci Appl 739, 239–246.
Annovazzi, L., Cattaneo, V., Viglio, S., Perani, E., Zanone, C., Rota, C., et al. (2004) High-performance liquid chromatography and capillary electrophoresis: methodological challenges for the determination of biologically relevant low-aliphatic aldehydes in human saliva. Electrophoresis 25, 1255–1263.
Herrington, J., Zhang, L., Whitaker, D., Sheldon, L., and Zhang, J.J. (2005) Optimizing a dansylhydrazine (DNSH) based method for measuring airborne acrolein and other unsaturated carbonyls. J Environ Monit 7, 969–976.
Seaman, V.Y., Charles, M.J., and Cahill, T.M. (2006) A sensitive method for the quantification of acrolein and other volatile carbonyls in ambient air. Anal Chem 78, 2405–2412.
Ho, S.S., and Yu, J.Z. (2004) Determination of airborne carbonyls: comparison of a thermal desorption/GC method with the standard DNPH/HPLC method. Environ Sci Technol 38, 862–870.
Slater, T.F. (1984) Overview of methods used for detecting lipid peroxidation. Meth Enzymol 105, 283–376.
Hicks, M., Delbridge, L., Yue, D.K., and Reeve, T.S. (1988) Catalysis of lipid peroxidation by glucose and glycosylated collagen. Biochem Biophys Res Commun 151, 649–655.
Steck, T.L., and Kant, J.A. (1974) Preparation of impermeable ghosts and inside out vesicles from human erythrocyte membranes. Meth Enzymol 31, 72–180.
Medina-Navarro, R., Mercado-Pichardo, E., Hernandez-Perez, O., and Hicks, J.J. (1999) Identification of acrolein from the ozone oxidation of unsaturated fatty acids. Hum Exp Toxicol 18, 677–682.
Medina-Navarro, R., Duran-Reyes, G., Diaz-Flores, M., Hicks, J.J., and Kumate, J. (2004) Glucose-stimulated acrolein production from unsaturated fatty acids. Hum Exp Toxicol 23, 101–105.
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Medina-Navarro, R. (2008). Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress I. Methods In Molecular Biology, vol 477. Humana Press. https://doi.org/10.1007/978-1-60327-517-0_13
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DOI: https://doi.org/10.1007/978-1-60327-517-0_13
Publisher Name: Humana Press
Print ISBN: 978-1-60327-218-6
Online ISBN: 978-1-60327-517-0
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