Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells

  • Anna-Katerina Hadjantonakis
  • Melinda Pirity
  • András Nagy
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

The introduction and establishment of transgenic, and in particular embryonic stem (ES) cell-based gene “knockout” technologies have made the mouse a key player in studying embryonic development and disease (1,2). In recent years, methods for the production of more complex genomic alterations have become increasingly widespread, hinting at an ability to manipulate and study a mammalian genome to an extent never previously thought possible. Such methodologies often partner homologous recombination-mediated gene targeting or random integration with site-specific recombination events.

This chapter is concerned with the utilization of the bacteriophage P1 derived site-specific recombinase protein Cre (3, 4, 5), and its employment as a means to catalyze modifications in homologously recombined and randomly integrated target sites within the mouse genome.

Cre is a 38-kDa protein that recombines DNA between two loxP target sites. loxP sequences are 34 basepairs (bp) long...


Embryonic Stem Embryonic Stem Cell Embryonic Stem Cell Line loxP Site Embryonic Stem Cell Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Anna-Katerina Hadjantonakis
    • 1
  • Melinda Pirity
    • 2
  • András Nagy
    • 2
  1. 1.Memorial Sloan Kettering Cancer CenterNew YorkUSA
  2. 2.Samuel Lunenfeld Research InstituteMount Sinai HospitalTorontoCanada

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