Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs
A pioneering experiment in the early 1980s demonstrated that microinjection of a recombinant growth hormone gene into the pronuclei of fertilized one-celled mouse embryos resulted in inheritable changes in the growth of the resulting “transgenic” mice and their offspring (1). Mammalian transgenic experiments have since contributed tremendously to our understanding of numerous com plex biological processes. The power of the technique lies in that it allows the function, and the developmental and physiological regulation, of almost any gene or protein to be studied within the context of the normal processes occur ring in the whole animal.
How DNA integrates into the host chromosome is not known, but some insights can be inferred from a study made on the state and organization of the inserts found in transgenic mice (2). Approximately 70% of the mice carry exogenous DNA in all their somatic and germ cells, implying that integration took place prior to the first round of...
KeywordsBody Wall Disposable Syringe Foster Mother Surrogate Mother Injection Chamber
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