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Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs

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Molecular Embryology

Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 461))

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References

  1. Palmiter RD, Brinster RL, Hammer RE, Trumbauer ME, Rosenfeld MG, Birberg NC et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothioneine-growth hormone fusion genes. Nature 300:611–615.

    Article  CAS  PubMed  Google Scholar 

  2. Palmiter RD, Brinster RL (1986) Germ line transformation of mice. Ann Rev Genet 20:465–499.

    Article  CAS  PubMed  Google Scholar 

  3. Lacy E, Roberts S, Evans EP, Burtenshaw MD, Constantini F (1983) A foreign beta globin gene in transgenic mice—integration at abnormal chromosomal positions and expression in inappropriate tissues. Cell 34:343–358.

    Article  CAS  PubMed  Google Scholar 

  4. Krumlauf R, Chapman VM, Hamme, RE, Brinster RL, Tilghman SM (1985) Differential expression of α-fetoprotein genes on the inactive X chromosome in extra-embryonic and somatic tissues of a transgenic rodent line. Nature 319:224–226.

    Article  Google Scholar 

  5. Wells T, Carter DA (2001) Genetic engineering of neural function in transgenic rodents: towards a comprehensive strategy? J Neurosci Methods 108:111–130.

    Article  CAS  PubMed  Google Scholar 

  6. Boyton RJ, Altmann DM (2002) Transgenic models of autoimmune disease. Clin Exp Immunol 127:4–11.

    Article  CAS  PubMed  Google Scholar 

  7. Wu X, Pandolfi PP (2001) Mouse models for multistep tumorigenesis. Trends Cell Biol 11:S2–S9.

    CAS  PubMed  Google Scholar 

  8. Waller SJ, Ho M, Murphy D (1995) In: Glover DM, Hames BD (eds) DNA Cloning 4: a practical approach IRL, New York, pp. 185–229.

    Google Scholar 

  9. Available at www.nap.edu/readingroom/books/labrats.

  10. Brinster RL, Chen HY, Trunbauer ME, Yagle MK, Palmiter RD (1985) Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc Natl Acad Sci USA 82:4438–4442.

    Article  CAS  PubMed  Google Scholar 

  11. Auerbach AB, Norinsky R, Ho W, Losos K, Guo Q, Chatterjee S, Joyner AL (2003) Strain-dependent differences in the efficiency of transgenic mouse production. Transgenic Research 12:59–69.

    Article  CAS  PubMed  Google Scholar 

  12. Vogelstein B, Gillespie D (1979) Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci USA 82:615–619.

    Article  Google Scholar 

  13. Constantini F, Lacy E (1981) Introduction of a rabbit B-globin gene into the mouse germ line. Nature 294:92–94.

    Article  Google Scholar 

  14. Leibo SP (1984) A one-step method for direct nonsurgical transfer of frozen-thawed bovine embryos. Theriogenology 21:767–790.

    Article  CAS  PubMed  Google Scholar 

  15. Pfaff RT, Agca Y, Liu J, Woods EJ, Peter AT, Critser JK (2000) Cryobiology of rat embryos, I: determination of zygote membrane permeability coefficients for water and cryoprotectants, their activation energies, and the development of improved cryopreservation methods. Biol Reprod 63:1294–1302.

    Article  CAS  PubMed  Google Scholar 

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Murphy, D. (2008). Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. METHODS IN MOLECULAR BIOLOGY™, vol 461. Humana Press. https://doi.org/10.1007/978-1-60327-483-8_7

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  • DOI: https://doi.org/10.1007/978-1-60327-483-8_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-431-9

  • Online ISBN: 978-1-60327-483-8

  • eBook Packages: Springer Protocols

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