Cell Grafting and Labeling in Postimplantation Mouse Embryos

  • A. Quinlan Gabriel
  • Poh-Lynn Khoo
  • Nicole Wong
  • A. Trainor Paul
  • P.L. Tam Patrick
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

Fate mapping experiments provide direct information on the differentiation pathways normally taken by cells or tissues during embryogenesis. Systematic analyses of the developmental fate of cell populations localized in different parts of the embryo enables the construction of fate maps. A comparison of the expression pattern of lineage-specific genes and the fate map allows the identification of precursor tissue for cell lineages well before definitive histogenesis takes place. The ability to trace the early lineage history of cells greatly facilitates the elucidation of the forces and processes that lead to the specification of cell lineages and the determination (or commitment) of cell fate. The knowledge of cell fate may also assist the interpretation of the phenotype of mutant embryos produced by either spontaneous mutation or gene knockout experiments.

This chapter describes the technical aspect of fate mapping the mouse embryo during gastrulation (6.5 days post...


Neural Crest Cell Germ Layer Tungsten Wire Primitive Streak Cell Clump 
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  1. 1.
    Lawson KA, Meneses JJ, Pedersen RA (1991).Clonal analysis of epiblast fate during germ layer formation in the mouse embryo. Development 113:891–911.PubMedGoogle Scholar
  2. 2.
    Quinlan GA, Williams EA, Tan S-S, Tam PPL (1995).Neuroectodermal fate of epiblast cells in the distal cap region of the mouse egg cylinder: implication for body plan organisation during early embryogenesis. Development 121:87–984.PubMedGoogle Scholar
  3. 3.
    Parameswaran M, Tam PPL (1995) Regionalisation of cell fate and morphogenetic movement of the mesoderm during mouse gastrulation. Develop Genet 17:16–28.CrossRefGoogle Scholar
  4. 4.
    Tam PPL, Beddington RSP (1987) The formation of mesodermal tissues in the mouse embryo during gastrulation and early organogenesis. Development 99:109–126.PubMedGoogle Scholar
  5. 5.
    Tam PPL, Beddington RSP (1992) Establishing and organisation of germ layers in the gastrulating mouse embryo. In: Postimplantation development in the mouse, Ciba Foundation Symp. 165. Wiley, Chichester, UK, pp. 27–49.Google Scholar
  6. 6.
    Tam PPL (1989) Regionalisation of mouse embryonic ectoderm: allocation of prospective ectodermal tissue during gastrulation. Development 107:55–67.PubMedGoogle Scholar
  7. 7.
    Trainor PA, Tan S-S, Tam PPL (1994) Cranial paraxial mesoderm: regionalisation of cell fate and impact upon craniofacial development in mouse embryos. Development 120:2397–2408.PubMedGoogle Scholar
  8. 8.
    Trainor PA, Tam PPL (1995) Cranial paraxial mesoderm and neural crest cells of the mouse embryo: co-distribution in the craniofacial mesenchyme but distinct segregation in branchial arches. Development 121:2569–2582.PubMedGoogle Scholar
  9. 9.
    Davidson BP, Tsang TE, Khoo P-L, Gad JM, Tam PPL (2003) Introduction of cell markers into germ layer tissues of the mouse gastrula by whole embryo electroporation. Genesis 35:57–62.CrossRefPubMedGoogle Scholar
  10. 10.
    Beddington RSP (1994) Induction of a second neural axis. Development 120:613– 620.PubMedGoogle Scholar
  11. 11.
    Miura S, Mishina Y (2003) Whole-embryo culture of E5.5 mouse embryos: development to the gastrulation stage. Genesis 37:38–43.CrossRefPubMedGoogle Scholar
  12. 12.
    Sturm K, Tam PPL (1992) Isolation and culture of whole postimplantation embryos and their germ layer derivatives. In: Wassarman P, DePamphilis M (eds) Methods in enzymology:. guide to techniques in mouse development. Academic Press, New York, pp. 164–190.Google Scholar
  13. 13.
    Jacobson AG, Tam PPL (1982) Cephalic neurulation in the mouse embryo analysed by SEM and morphometry. Anat Rec 203:375–395.CrossRefPubMedGoogle Scholar
  14. 14.
    Chan WY, Tam PPL (1988) A morphological and experimental study of the mesen-cephalic neural crest cells in the mouse embryo using wheat germ agglutinin-gold conjugates as the cell marker. Development 102:427–442.PubMedGoogle Scholar
  15. 15.
    Serbedzija GN, Bronner-Fraser M, Fraser SE (1989) A vital dye analysis of timing and pathways of avian neural crest cell migration. Development 106:809–816.PubMedGoogle Scholar
  16. 16.
    Kinder SJ, Tan S-S, Tam PPL (2000). Cell grafting and fate mapping of the early-somite-stage mouse embryo. In: Methods in molecular biology, vol.1, pp. 425–437, Developmental Biology Protocols, vol. 135.Google Scholar

Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • A. Quinlan Gabriel
    • 1
  • Poh-Lynn Khoo
    • 1
  • Nicole Wong
    • 1
  • A. Trainor Paul
    • 2
  • P.L. Tam Patrick
    • 1
  1. 1.Embryology Unit, Children's Medical Research InstituteUniversity of SydneySydneyAustralia
  2. 2.Stowers Institute, Kansas CityKansasUSA

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