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Molecular Embryology pp 735–744Cite as

Protein Techniques

Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting

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Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 461))

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References

  1. Koopman, P., Gubbay, J., Vivian, N., Goodfellow, P. N., and Lovell-Badge, R. (1991) Male development of chromosomally female mice transgenic for Sry. Nature 351, 117–127.

    Article  CAS  PubMed  Google Scholar 

  2. Bloom, T. and McConnell, J. (1990) Changes in protein phosphorylation associated with compaction of the mouse preimplantation embryo. Mol. Reprod. Dev. 26, 199–210.

    Article  CAS  PubMed  Google Scholar 

  3. Fantl, W. J., Johnson, D. E., and Williams, L. T. (1993) Signalling by receptor tyro-sine kinases. Ann. Rev. Biochem. 62, 453–481.

    CAS  PubMed  Google Scholar 

  4. Wassarman, D. A., Therrien, M., and Rubin, G. M. (1995) The ras signalling pathway in Drosophila. Curr. Opin. Genet. Dev. 5, 44–50.

    Article  CAS  PubMed  Google Scholar 

  5. Dickson, B. (1995) Nuclear factors in sevenless signalling. Trends Genet. 11, 106–111.

    Article  CAS  PubMed  Google Scholar 

  6. Itoh, K. and Sokol, S. Y. (1994) Heparan sulfate proteoglycans are required for mesoderm formation in Xenopus embryos. Development 120, 2703–2711.

    CAS  PubMed  Google Scholar 

  7. Brown, T. L., Fisher, W. C., Collins, M. D., De, B. K., and Scott, W. J., Jr. (1994) Identification of a 100-kDa Phosphoprotein in developing murine embryos as Elongation Factor 2. Arch. Biochem. Biophys. 309, 105–110.

    Article  CAS  PubMed  Google Scholar 

  8. Schneider, D. S., Jin, Y., Morisato, D., and Anderson, K. V. (1994) A processed form of the Spätzle protein defines dorsal-ventral polarity in the Drosophila embryo. Development 120, 1243–1250.

    CAS  PubMed  Google Scholar 

  9. Mathieu, M., Kiefer, P., Mason, I., and Dickson, C. (1995) Fibroblast Growth Factor (FGF) 3 from Xenopus Laevis (XFGF3) binds with high affinity to FGF receptor 2. J. Biol. Chem. 270, 6779–6787.

    Article  CAS  PubMed  Google Scholar 

  10. Laemmli, U. K. (1970) Cleavage of the structural protein during the assembly of the head of the bacteriophage T4. Nature 227, 680–685.

    Article  CAS  PubMed  Google Scholar 

  11. Sambrook, J., Fritsch, E. F., and Maniatis, T. (eds.) (1989) Molecular Cloning. A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N Y.

    Google Scholar 

  12. James, G. T. (1978) Inactivation of the protease inhibitor phenylmethylsulphonyl flouride in buffers. Anal. Biochem. 86, 574–579.

    Article  CAS  PubMed  Google Scholar 

  13. Lazarovitz, A. I., Osman, N., Le Feuvre, C., Ley, S. C., and Crumpton, M. J. (1994) CD7 is associated with CD3 and CD45 on Human T cells. J. Immunol. 3957–3966.

    Google Scholar 

  14. Jackson, D. I., Verbi, W., Lazarovitz, A. I., and Crumpton, M. J. (1995) T lymphocyte activation: The role of tyrosine phosphorylation. Cancer Surveys 22, 97–110.

    CAS  PubMed  Google Scholar 

  15. Schneider, C., Newman, R. A., Sutherland, D. R., Asser, U., and Greaves, M. F. (1982) A one-step purification of membrane proteins using a high efficiency immu-nomatrix. J. Biol. Chem. 257, 10,766–10,769.

    CAS  Google Scholar 

  16. Aghion, J., Gueth-Hallonet, C., Antony, C., Gros, D., and Maro, B. (1994) Cell adhesion and gap junction formation in the early mouse embryo are induced prematurely by 6-DMAP in the absence of E-cadherin phosphorylation. J. Cell. Sci. 107, 1369–1379.

    CAS  PubMed  Google Scholar 

  17. Christians, E., Campion, E., Thompson, E. M., and Renard, J-P. (1995) Expression of the HSP 70.1 gene, a landmark of early zygotic activity in the mouse embryo, is restricted to the first burst of transcription. Development 121, 113–122.

    CAS  PubMed  Google Scholar 

  18. Fagotto, F. and Gumbiner, B. M. (1994) ̫-Catenin localisation during Xenopus embryogenesis: accumulation at tissue and somite boundaries. Development 120, 3667–3679.

    CAS  PubMed  Google Scholar 

  19. Hames, B. D., and Rickwood, D., eds. (1981) Gel Electrophoresis of Proteins—A Practical Approach. IRL Press, Oxford University Press, Oxford, UK.

    Google Scholar 

  20. Sammons, D. W., Adams, L. D., and Nishizawa, E. E. (1981) Ultrasensitive silver-based color staining of polypeptides in polyacylamide gels. Electrophoresis 2, 135.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Retired, Cancer Research UK, London, UK

    Clive Dickson

Authors
  1. Clive Dickson
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Editor information

Editors and Affiliations

  1. Department of Craniofacial Development, King's College London, London, UK

    Paul T. Sharpe

  2. MRC Centre for Developmental Neurobiology, King's College London, London, UK

    Ivor Mason

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© 2008 Springer Science + Business Media, LLC

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Dickson, C. (2008). Protein Techniques. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. METHODS IN MOLECULAR BIOLOGY™, vol 461. Humana Press. https://doi.org/10.1007/978-1-60327-483-8_53

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  • DOI: https://doi.org/10.1007/978-1-60327-483-8_53

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-431-9

  • Online ISBN: 978-1-60327-483-8

  • eBook Packages: Springer Protocols

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