In-Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections
The development of such techniques as transgenesis, saturation mutagenesis, and the polymerase chain reaction (PCR), all of which are described in detail elsewhere in this volume, revolutionized experimental embryology. However, no single procedure has been applied more broadly across the field than that of in-situ hybridization to RNA. This allowed researchers to determine rapidly the spatial and temporal expression of their gene of interest without having to resort to more tedious and less certain approaches requiring the production of antis-era against the protein product. As such, in-situ hybridization has become the standard first step toward the characterization of the developmental significance of a newly identified gene. Of course, in-situ hybridization tells you nothing regarding the translation of the mRNA into protein or about protein's subsequent localization, modification, or stability. However, in-situ hybridization remains an extremely powerful tool,...
KeywordsFume Hood Large Material DEPC Water Small Embryo Fume Cupboard
Research in the Ivor Mason's laboratory is supported the MRC, BBSRC, and the Wellcome Trust; and he is a research fellow of the Leverhulme Trust.
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