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RT-PCR on Embryos Using Degenerate Oligonucleotide Primers

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Molecular Embryology

Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 461))

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References

  1. Sambrook, J., Fritsch, E. F., and Maniatis, T. (eds.) (1989)Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N Y. 666

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  2. Chomczynski, P. and Sacchi, N. (1987) Single step method of RNA isolation by guanindinium thiocyanate-phenol-chloroform extraction.Anal. Biochem. 162, 156–159.

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  3. Childs, S. R., Wrana, J. L., Arora, K., Attisano, L., O'Connor, M. B., and Massague, J. (1993) Identification of aDrosophila activin receptor.Proc. Natl. Acad. Sci. USA 90, 9475–9479.

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Graham, A. (2008). RT-PCR on Embryos Using Degenerate Oligonucleotide Primers. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. METHODS IN MOLECULAR BIOLOGY™, vol 461. Humana Press. https://doi.org/10.1007/978-1-60327-483-8_43

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  • DOI: https://doi.org/10.1007/978-1-60327-483-8_43

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-431-9

  • Online ISBN: 978-1-60327-483-8

  • eBook Packages: Springer Protocols

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