Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)
Using DNA Microarrays
Expression profiling using DNA microarrays offers a high-throughput, semi-quantitative method for studying the transcriptional basis of development in metazoans and, at the same time, affords a novel approach to functional annotation of the genome. The increasingly inventive use of microarrays to be found in the literature is testimony to their growing impact in the biosciences. However, for developmental biologists their exploitation (reviewed in ( 1)) has some well-characterized impediments, perhaps paramount among these being the limiting amounts of RNA available and the paucity of affordable, high-quality, genomewide microarrays. Notwithstanding these obstacles, which are gradually becoming less significant, we describe a series of protocols that have been used in our laboratory in a wide range of experimental systems, including developmental models. The description of these protocols rests on the following assumptions and points:
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KeywordsSodium Dodecyl Sulphate Wash Solution Saline Sodium Citrate Target Solution RNeasy Column
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We thank Helen Hilton for helpful comments on the manuscript and Steve Thomas for the artwork.
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© Humana Press, a part of Springer Science + Business Media, LLC 2008