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References
Welsh, J. and McClelland, M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18, 7213–7218.
Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingey, S. V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531–6535.
Welsh, J., Chada, K., Dalal, S. S., Ralph, D., Chang, R., and McClelland, M. (1992) Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Res. 20, 4965– 4970.
Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–971.
Welsh, J., Chada, K., Dalal, S. S., Cheng, R., Ralph, D., and McClelland, M. (1992) Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Res. 20, 4965–4970.
Ralph, D., McClelland, M., and Welsh, J. (1993) RNA fingerprinting using arbitrarily primed PCR identifies differentially regulated RNAs in mink lung (My1Lu) cells growth arrested by transforming growth factor b1. Proc.Natl.Acad.Sci. USA 90, 10,710–10,714.
Liang, P., Averboukh, L., Keyomarsi, K., Sager, R., and Pardee, A. B. (1992) Differential display and cloning of messenger RNAs from human breast cancer versus mammary epithelial cells. Cancer Res. 52, 6966–6968.
Liang, P., Zhu, W., Zhang, X., Guo, Z., O'Connell, R. P., Auerboukh, L., Wang, F., and Pardee, A. B. (1994) Differential display using one-base anchored oligodT. Nucleic Acids Res. 25, 5763,5764.
Zimmermann, J. W. and Schultz, R. M. (1994) Analysis of gene expression in the preimplantation mouse embryo: use of mRNA differential display. Proc. Natl. Acad. Sci. USA. 91, 5456–5460.
Loh, E. Y., Elliot, J. F., Cwirla, S., Lanier, L. L., and Davis, M. M. (1989) Polymer-ase chain reaction with single-sided specificity: Analysis of T cell receptor d chain. Science 243, 217–220.
Chomczynski, P. and Sacchi, N. (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem 162, 156–159.
Feinberg, A. P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13.
Li, F., Barnathan, E. S., and KarikĂł, K. (1994) Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of Northern blot for affinity capturing of cDNAs. Nucleic Acids Res. 22, 1764,1765.
Honeycutt, R., Sobral, B. W., and McClelland, M. (1997) Polymerase chain reaction (PCR) detection and quantification using a short PCR product and a synthetic internal positive control. Anal. Biochem. 248, 303–306.
Sambrook, J., Fritsh, E. F., and Maniatis, T. (eds.) (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Guimaraes, M. J., Lee, F., Zlotnik, A., and McClanahan, T. (1995) Differential display by PCR: novel findings and applications. Nucleic Acids Res. 23, 1832,1833.
Acknowledgments
This work was supported by Fundacion Cellex, the G. Harold and Leila Y. Mathers Charitable Foundation, Marato and MEC.
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Tugores, A., Belmonte, J.C.I. (2008). Differential Display of Eukaryotic mRNA. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. METHODS IN MOLECULAR BIOLOGY™, vol 461. Humana Press. https://doi.org/10.1007/978-1-60327-483-8_40
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DOI: https://doi.org/10.1007/978-1-60327-483-8_40
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