Differential Display of Eukaryotic mRNA

  • Antonio Tugores
  • Juan Carlos Izpisua Belmonte
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

Arbitrarily primed PCR is a method that was initially developed to analyze and compare genome complexity (1,2). By using arbitrary primers, an array of stochastic sequences can be amplified using the polymerase chain reaction (PCR) and resolved by denaturing acrylamide gels, generating a characteristic pattern or fingerprint. This technique can be readily applied for the analysis of differentially expressed genes in two different cell types by simply converting the mRNA into cDNA (3,4). A different fingerprint pattern will then reflect differential gene expression between the cell types analyzed. The number and intensity of the fingerprints depend largely on two parameters: the abundance of the original RNAs, and how well the primers match the primary sequence so that efficient amplification can take place. Taking this into consideration, the method appears to be more suitable for the comparison of cell types where a large number of differentially expressed genes are...


Sodium Dodecyl Sulfate Polymerase Chain Reaction Product Xylene Cyanol Ethidium Bromide Solution Polymerase Chain Reaction Tube 
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This work was supported by Fundacion Cellex, the G. Harold and Leila Y. Mathers Charitable Foundation, Marato and MEC.


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Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Antonio Tugores
    • 1
  • Juan Carlos Izpisua Belmonte
    • 1
    • 2
  1. 1.Gene Expression Laboratory, Stem Cell Research Center, and Laboratory of GeneticsThe Salk Institute for Biological StudiesLa JollaUSA
  2. 2.The Center of Regenerative Medicine in BarcelonaBarcelonaSpain

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