Treatment of Mice with Retinoids In Vivo and In Vitro
The retinoids comprise a large group of natural and synthetic compounds related to vitamin A (retinol). The family name is derived from the early observation of the necessity of vitamin A for normal vision and the association of vitamin A deficiency with night blindness (1). With the exception of the visual cycle in the rod photoreceptor cells of the retina, in which protein-bound 11-cis-retinal is reversibly isomerized to free all-trans-retinal, retinoic acid is the active retinoid for biological processes. However, the visual cycle illustrates two characteristics of retinoids that are relevant to the use of this family of molecules as tools in experimental embryology and for the interpretation of results: (1e light-sensitive, and will undergo isomerization or degradation if exposed to light; (2) in nature, their activity and their metabolism are associated with binding to specific proteins.
The most important natural retinoids are retinyl esters, retinol, and retinoic...
KeywordsRetinoic Acid Retinol Binding Protein Retinyl Ester Retinyl Acetate Limb Abnormality
- 1.Moore T (1957) Vitamin A. Elsevier, Amsterdam.Google Scholar
- 2.Mangelsdorf DJ, Umesono K, Evans RM (1994) The retinoid receptors. In: Sporn MB, Roberts AB, Goodman DS (eds) The retinoids: biology, chemistry and medicine (2nd ed,). Raven, New York, pp. 319–349.Google Scholar
- 5.Nau H (1994) Retinoid teratogenesis: toxicokinetics and structure specificity. In: Bolt HM, Hellman B, Denker L (eds) Use of mechanistic information in risk assessment (Archives in Toxicology, Supplement 16), Springer-Verlag, Berlin, pp. 118–127.Google Scholar
- 9.Creech-Kraft J, Löfberg B, Chahoud I, Bochert G, Nau H (1989) Teratogenicity and placental transfer of all-trans-, 13-cis-, 4-oxo-all-trans-, and 4-oxo-13-cis-retinoic acid after administration of a low oral dose during organogenesis in mice. Toxicol Appl Pharmacol 100:162–176.CrossRefPubMedGoogle Scholar
- 15.Chen W-H, Morriss-Kay GM, Copp A (l995) Genesis and prevention of spinal neural tube defects in the curly tail mutant mouse: involvement of retinoic acid and its nuclear receptors RAR-£\ and RAR-£ ˆ. Development 121:681–691.Google Scholar