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Small-Scale Marker-Based Screening for Mutations in Zebrafish Development

  • D. Currie Peter
  • F. Schilling Thomas
  • W. Ingham Philip
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

We describe a standardized mutagenic protocol and a methodology for small-scale directed screening of the zebrafish genome for mutations in specific developmental processes. The methods are based primarily on those developed for large-scale screens in Tubingen, Germany; Boston, MA; and Eugene, OR as well as our experiences with a smaller facility. By combining a marker-based screening protocol with both haploid and diploid screening methods, one can efficiently recover mutants in specific processes.

Random mutagenesis provides the ability to survey the genome of an organism, without bias, for genes that function in particular processes. For many years, geneticists have been reaping the rich harvest of mutations produced by such a mutagenic approach, directed against particular developmental processes of the fruit fly Drosophila melanogaster (1). Analysis of the genes uncovered by this approach has revolutionized our understanding of the genetic control of animal...

Keywords

Early Pressure Alcian Blue Zebrafish Embryo Zebrafish Genome Haploid Embryo 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • D. Currie Peter
    • 1
  • F. Schilling Thomas
    • 2
  • W. Ingham Philip
    • 3
  1. 1.Victor Chang Cardiac Research InstituteVictor Chang Cardiac Research InstituteSydneyAustralia
  2. 2.Department of Developmental and Cell BiologyUniversity of California, IrvineCaliforniaUSA
  3. 3.Centre for Developmental GeneticsUniversity of SheffieldSheffieldUK

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