Organ Culture in the Analysis of Tissue Interactions

  • Irma Thesleff
  • Carin Sahlberg
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

Interactions between epithelial and mesenchymal tissues constitute a central mechanism regulating the development of most embryonic organs. Studies on the nature of such interactions require the separation of the interacting tissues from each other and the follow-up of their advancing development in various types of recombined explants. The tissues can be either transplanted and their development followed in vivo or cultured as explants in vitro.

Although the transplantation methods offer certain advantages, including a physiological environment and the possibility for long-term follow-up, organ culture techniques are superior in many other aspects. The cultured tissues can be manipulated in multiple ways, and their development can be continuously monitored. The culture conditions are reproducible, and the composition of the medium is known exactly and can be modified. Furthermore, the in vitro culture conditions allow analyses of the nature of the inductive signals.



Tooth Germ Glass Petri Dish Metal Grid Nuclepore Filter Disposable Needle 
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Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Irma Thesleff
    • 1
  • Carin Sahlberg
    • 1
  1. 1.Institute of BiotechnologyUniversity of HelsinkiHelsinkiFinland

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