Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)
Mesoderm Induction Assays
Inductive interactions play a major role in early development, and one of the earliest such interactions in amphibian development, and perhaps the in development of all vertebrates, is mesoderm induction ( 1, 2, 3, 4, 5). Mesoderm induction occurs at blastula stages, when a signal from the vegetal hemisphere of the embryo acts on overlying equatorial cells, causing them to form mesoderm rather than ectoderm. This interaction was first discovered in experiments in which prospective ectodermal tissue of the embryo (the so-called “animal cap”) is juxtaposed with future endoderm from the vegetal hemisphere (Fig. 1). When cultured alone, the animal caps form epidermis; when cultured adjacent to vegetal pole blastomeres, they form mesoderm.
KeywordsHuman Chorionic Gonadotrophin Xenopus Embryo Animal Pole Pregnant Mare Serum Gonadotrophin Vegetal Pole
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- 13.Smith, J. C. (1993) Purifying and assaying mesoderm-inducing factors from vertebrate embryos, in: Cellular Interactions in Development—A Practical Approach (Hartley, D., ed.), Oxford University Press, Oxford, UK, pp. 181–204.Google Scholar
- 18.Symes, K. and Smith, J. C. (1987) Gastrulation movements provide an early marker of mesoderm induction in Xenopus. Development 101,339–349.Google Scholar
- 19.Green, J. B. A., Howes, G., Symes, K., Cooke, J., and Smith, J. C. (1990) The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF. Development 108,229–238.Google Scholar
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