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Electroporation in Avian Embryos

  • Jun-ichi Funahashi
  • Harukazu Nakamura
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

Manipulating the gene expression is a powerful approach to study its function. Here, we introduce in-ovo electroporation as a very effective gene transfer method. By this method, one can test gene function quickly and easily. Gain-of-function study of the gene of interest by this method greatly contributed to understand the molecular mechanisms of development ( 1, 2, 3). Gene silencing by electroporating the siRNA vector is now possible (( 4) see also Note 1). We have to construct only an appropriate expression vector, locally apply this plas-mid to an avian embryo, and charge electric pulses. The translated product is observed only 2 h after electroporation (Fig. 1). The area of transfection can be localized or spread by choice of electrode. There are also drawbacks, when it is compared with the retrovirus-mediated gene transfer. The expression with this method is transient, and not all of the cells facing to the electrode can be trans-fected as far as when the...

Keywords

Needle Electrode Vitelline Membrane Avian Embryo Parallel Electrode Tungsten Needle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Jun-ichi Funahashi
    • 1
  • Harukazu Nakamura
    • 1
  1. 1.Department of Molecular Neurobiology, Institute of Development, Aging and CancerTohoku UniversitySendaiJapan

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