Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)
Electroporation in Avian Embryos
Manipulating the gene expression is a powerful approach to study its function. Here, we introduce in-ovo electroporation as a very effective gene transfer method. By this method, one can test gene function quickly and easily. Gain-of-function study of the gene of interest by this method greatly contributed to understand the molecular mechanisms of development ( 1, 2, 3). Gene silencing by electroporating the siRNA vector is now possible (( 4) see also Note 1). We have to construct only an appropriate expression vector, locally apply this plas-mid to an avian embryo, and charge electric pulses. The translated product is observed only 2 h after electroporation (Fig. 1). The area of transfection can be localized or spread by choice of electrode. There are also drawbacks, when it is compared with the retrovirus-mediated gene transfer. The expression with this method is transient, and not all of the cells facing to the electrode can be trans-fected as far as when the...
KeywordsNeedle Electrode Vitelline Membrane Avian Embryo Parallel Electrode Tungsten Needle
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