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Quail—Chick Chimeras

  • Marie-Aimée Teillet
  • Catherine Ziller
  • Nicole M. Le Douarin
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

The understanding of several mechanisms that are essential for embryonic development has greatly benefited from cell-marking techniques that allow tracing of definite cells and their progeny, and thus, the study of their behavior and fate. A cell marker must be precise and stable; it must not interfere with normal development. The quail—chick labeling technique meets these requirements perfectly.

The principle of the method ( 1) is based on the observation that in all embryonic and adult cells of the quail ( Coturnix coturnix japonica), the heterochromatin is condensed in one (sometimes two or more, depending on the cell types) large mass(es) associated with the nucleolus, thus making this organelle strongly stained after DNA staining, e.g., the Feulgen and Rossenbeck staining ( 2). When quail cells are combined with cells of the chick ( Gallus gallus) which possess, like most of the animal cells, only small chromocenters dispersed in the nucleoplasm, they are readily...

Keywords

Neural Tube Neural Crest Cell Tyrode Solution Donor Embryo Brain Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Marcelle Gendreau and Charmaine Herberts for reference filing, Fran殩se Viala for photographic work, and Sophie Gournet and Hélène San Clemente for line drawings.

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Marie-Aimée Teillet
    • 1
  • Catherine Ziller
    • 1
  • Nicole M. Le Douarin
    • 1
  1. 1.Instituit d'Embryologie Cellulaire et MoleculaireCollege de FranceNogent-sur-MarneFrance

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