Tissue Recombinations in Collagen Gels

  • Marysia Placzek
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

The characterization of molecular and antigenic markers of cells in many vertebrate tissues has increased dramatically in recent years, allowing cell differentiation and organogenesis to be followed in vivo in a way that was unprecedented even two decades ago. In-vitro systems that allow tissues and organs to be isolated and cultured can be used to great effect in parallel with in-vivo studies, to dissect the tissue interactions, signaling and transcription factors that direct tissue patterning, cell differentiation, and organogenesis. Three-dimensional collagen gels provide an excellent in-vitro culture environment. First, they provide a scaffold that supports tissue architecture, a requirement vital for proper development of any three-dimensional tissue or organ system. Second, they stabilize diffusible gradients. Initially developed as a means to culture embryonic neuronal tissue and examine the effect of trophic factors (1), such gels were then initially used to...


Explanted Tissue Tungsten Needle Remove Culture Medium Collagen Preparation Hanging Drop Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Ebendal T, Jacobson CO (1977) Tissue explants affecting extension and orientation of axons in cultured chick embryo ganglia. Exper Cell Res 105:379–387.CrossRefGoogle Scholar
  2. 2.
    Basler K, Edlund T, Jessell TM, Yamada T (1993) Control of cell pattern in the neural tube: regulation of cell differentiation by dorsalin-1, a novel TGFbeta family member. Cell 73:687–702.CrossRefPubMedGoogle Scholar
  3. 3.
    Fan CM, Tessier-Lavigne M (1994) Patterning of mammalian somites by surface ectoderm and notochord: evidence for sclerotome induction by a hedgehog homolog. Cell 79:1175–1186.CrossRefPubMedGoogle Scholar
  4. 4.
    Roelink H, Augsburger A, Heemskerk J, Korzh V, Norlin S, Ruiz i Altaba A, Tanabe Y, Placzek M, Edlund T, Jessell TM (1994) Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord. Cell 76:761–775.CrossRefPubMedGoogle Scholar
  5. 5.
    Tessier-Lavigne M, Placzek M, Lumsden AGS, Dodd J, Jessell TM (1988) Chemo-tropic guidance of developing axons in the mammalian central nervous system. Nature 336:775–778.CrossRefPubMedGoogle Scholar
  6. 6.
    Serafini T, Kennedy TE, Galko MJ, Mirzayan C, Jessell TM and Tessier-Lavigne M (1994) The netrins define a family of axon outgrowth-promoting proteins homologous to C. elegans UNC-6. Cell 78:409–24.CrossRefPubMedGoogle Scholar
  7. 7.
    Serafini T, Kennedy TE, Galko MJ, Mirzayan C, Jessell TM, Tessier LM (1994) The netrins define a family of axon outgrowth-promoting proteins homologous to C. elegans UNC-6. Cell 78:409–424.CrossRefPubMedGoogle Scholar
  8. 8.
    Heffner CD, Lumsden AGS, O'Leary DDM (1990) Target control of collateral extension and directional axon growth in the mammalian brain. Science 247:217–247.CrossRefPubMedGoogle Scholar
  9. 9.
    Lumsden AGS, Davies AM (1983) Earliest sensory nerve fibres are guided to peripheral targets by attractants other than nerve growth factor. Nature 306: 786–788.CrossRefPubMedGoogle Scholar
  10. 10.
    Messersmith EK, Leonardo ED, Shatz C, Tessier-Lavigne M, Goodman C S, Kolodkin AL (1995) Semaphorin III can function as a selective chemorepellant to pattern sensory projections in the spinal cord. Neuron 14:949–959.CrossRefPubMedGoogle Scholar
  11. 11.
    Sanvito F, Herrera PL, Huarte J, Nichols A, Montesano R, Orci L, Vassalli JD (1994) TGF-beta 1 influences the relative development of the exocrine and endocrine pancreas in vitro. Development 120:3451–62.PubMedGoogle Scholar
  12. 12.
    Larsen MC, Brake PB, Pollenz RS, Jefcoate CR. (2004) Linked expression of Ah receptor, ARNT, CYP1A1, and CYP1B1 in rat mammary epithelia, in vitro, is each substantially elevated by specific extracellular matrix interactions that precede branching morphogenesis. Toxicol Sci 82:46–61.CrossRefPubMedGoogle Scholar
  13. 13.
    Greenberg S, Margulis A, Garlick JA (2004) In vivo transplantation of engineered human skin. Methods Mol Biol 289:425–430Google Scholar
  14. 14.
    Doillon CJ, Gagnon E, Paradis R, Koutsilieris M (1994). Three-dimensional culture system as a model for studying cancer cell invasion capacity and anticancer drug sensitivity. Anticancer Res 24:2169–2177.Google Scholar

Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Marysia Placzek
    • 1
  1. 1.Centre for Developmental GeneticsUniversity of SheffieldSheffieldUK

Personalised recommendations