Tissue Recombinations in Collagen Gels
The characterization of molecular and antigenic markers of cells in many vertebrate tissues has increased dramatically in recent years, allowing cell differentiation and organogenesis to be followed in vivo in a way that was unprecedented even two decades ago. In-vitro systems that allow tissues and organs to be isolated and cultured can be used to great effect in parallel with in-vivo studies, to dissect the tissue interactions, signaling and transcription factors that direct tissue patterning, cell differentiation, and organogenesis. Three-dimensional collagen gels provide an excellent in-vitro culture environment. First, they provide a scaffold that supports tissue architecture, a requirement vital for proper development of any three-dimensional tissue or organ system. Second, they stabilize diffusible gradients. Initially developed as a means to culture embryonic neuronal tissue and examine the effect of trophic factors (1), such gels were then initially used to...
KeywordsExplanted Tissue Tungsten Needle Remove Culture Medium Collagen Preparation Hanging Drop Culture
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