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EC Culture

A Method to Culture Early Chick Embryos
  • Andrea Streit
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

Avian embryos have been used as a model system to study development for more than two centuries and have proven to be particularly useful to study events that occur at early developmental stages, when other amniote species are difficult to access and maintain in vitro. They lend themselves to embryo-logical manipulation, such as interspecies tissue transplantation or cell lineage analysis as well as observation of cellular behavior in the living embryo. At the time of egg laying, the embryo is a large, translucent disc of approximately 50,000 cells that is easily accessible and develops well in vitro from before primitive streak formation. Two well-defined staging systems are available that describe preprimitive streak stages (using Roman numbers I–XIV [1]) and the stages after primitive streak formation (in Arabic numbers 2–46 [2]). With recent technical advances, like introduction of expression constructs, antisense RNA (morpholinos), and dsRNA by electroporation,...

Keywords

Chick Embryo Ventral Side Primitive Streak Vitelline Membrane Glass Ring 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

I thank Andrew Bailey for assistance with photography and Claudio Stern for useful comments on the manuscript.

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Andrea Streit
    • 1
  1. 1.Department of Craniofacial DevelopmentKing's College LondonLondonUK

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