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EC Culture

A Method to Culture Early Chick Embryos

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Molecular Embryology

Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 461))

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References

  1. Eyal-Giladi H, Kochav S (1976) From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick. Devl Biol 49:321–337.

    Article  CAS  Google Scholar 

  2. Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick. J Morph 88:49–92.

    Article  Google Scholar 

  3. McWhorter JE, Whipple HO (1912) The development of the blastoderm of the chick in vitro. Anat Rec 6:121–140.

    Article  Google Scholar 

  4. Waddington CH (1932) Experiments on the development of chick and duck embryos, cultivated in vitro. Phil Trans Roy Soc Lond B. 221:179–230.

    Article  Google Scholar 

  5. Spratt NT (1947a) Development in vitro of the early chick blastoderm explanted on yolk and albumen extract saline-agar substrata. J Exp Zool 106:345–366.

    Article  Google Scholar 

  6. Spratt NT (1947b) A simple method for explanting and cultivating early chick embryos in vitro. Science 106:452.

    Article  Google Scholar 

  7. Spratt NT, Haas H (1960a) Morphogenetic movements in the lower surface of the unincubated and early chick blastoderm. J Exp Zool 144:139–157.

    Article  Google Scholar 

  8. Spratt NT, Haas H (1960b) Integrative mechanisms in development of the early chick blastoderm, I. Regulative potentiality of separated parts. J Exp Zool 145:97–137.

    Article  Google Scholar 

  9. De Haan RL (1967) Avian embryo culture. In: Wilt FH, Wessels NK (eds) Methods in developmental biology. Thomas Y. Crowell, New York, pp. 401–412.

    Google Scholar 

  10. New DAT (1955) A new technique for the cultivation of the chick embryo in vitro. J Embryol Exp Morph 3:326–331.

    Google Scholar 

  11. Stern CD (1993). Avian embryos. In: Stern CD, Holland PWH (eds) Essential developmental biology: a practical approach. IRL Press at Oxford University Press, Oxford, UK, pp. 45–54.

    Google Scholar 

  12. Stern CD, Bachvarova R (1997) Early chick embryos in vitro. Int J Develop Biol 41:379–387.

    CAS  Google Scholar 

  13. Nicolet G, Gallera J. (1961) Quelques commentaires sur les méthodes de culture in vitro de jeunes blastodermes de poulet. Experientia 17:134–135.

    Article  PubMed  Google Scholar 

  14. De Carli HO (1965) Acción de la heparina sobre el tubo neural del embrión de pollo: Nueva técnica de cultivo de embrión de pollo in vitro. Rev Soc Argent Biol 41:19–23.

    CAS  PubMed  Google Scholar 

  15. Kučera, P, Burnand M-B (1987) Routine teratogeniCity test that uses chick embryos in vitro. Teratogenesis, Carcinogenesis and Mutagenesis 7:427–447.

    Article  Google Scholar 

  16. Seidl W (1977) Description of a device facilitating the in vitro culture of chick embryo according to the New method. Folia Morphologica (Praha) 25:43–45.

    CAS  Google Scholar 

  17. Stern CD, Ireland GW (1981) An integrated experimental study of endoderm formation in avian embryos. Anat Embryol 163:245–263.

    Article  CAS  PubMed  Google Scholar 

  18. Connolly D, McNaughton LA, Krumlauf R, Cooke J (1995) Improved in vitro development of the chick embryo using roller-tube culture. Trends Genet 11:259–260.

    Article  CAS  PubMed  Google Scholar 

  19. New DAT (1959) The adhesive properties and expansion of the chick blastoderm. J Embryol Exp Morph 7:146–164.

    CAS  PubMed  Google Scholar 

  20. Stern CD, Bellairs R (1984) The roles of node regression and elongation of the area pellucida in the formation of somites in avian embryos. J Embryol Exp Morph 81:75–92.

    CAS  PubMed  Google Scholar 

  21. Chapman SC, Collignon J, Schoenwolf FC, Lumsden A (2001) Improved method for chick whole-embryo culture using a filter paper carrier. Dev Dyn 220:284–289.

    Article  CAS  PubMed  Google Scholar 

  22. Pannett CA, Compton A (1924). The cultivation of tissues in saline embryonic juice. Lancet 206:381–384.

    Article  Google Scholar 

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Acknowledgments

I thank Andrew Bailey for assistance with photography and Claudio Stern for useful comments on the manuscript.

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© 2008 Humana Press, a part of Springer Science + Business Media, LLC

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Streit, A. (2008). EC Culture. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. METHODS IN MOLECULAR BIOLOGY™, vol 461. Humana Press. https://doi.org/10.1007/978-1-60327-483-8_17

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  • DOI: https://doi.org/10.1007/978-1-60327-483-8_17

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-431-9

  • Online ISBN: 978-1-60327-483-8

  • eBook Packages: Springer Protocols

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