Advertisement

New Culture

  • Amata Hornbruch
Protocol
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

As the title indicates, this culture method was developed by Denis New and first described in 1955 (1). It enables the observer to study the events of gastru-lation in the chick embryo in much greater detail than possible until then. It also opens the way to microsurgery without the problem that yolk and the vitelline membrane cause. Studying the effects of treatment with compound affecting morphogenesis and development became meaningful, because precise concentrations and volumes could be administered and successfully washed out again.

Most of the early work on the mechanics of gastrulation was performed on the egg of Xenopus laevis, because of its accessibility. New Culture offered a chance to emulate and refine some of those experiments in the chick embryo.

Several attempts have been made to culture blastoderms or fragments thereof in vitro, but none have been very successful. The chorioallantoic membrane (CAM grafts) has been used as a substratum to study...

Keywords

Chick Embryo Balance Salt Solution Watch Glass Vitelline Membrane Morphogenetic Movement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    New, D. A. T. (1955) A new technique for the cultivation of the chick embryo in vitro. J. Embryol. Exp. Morphol. 3, 326–331.Google Scholar
  2. 2.
    Spratt, N. T., Jr. (1947) A simple method for explanting and cultivating early chick embryos in vitro. Science 106, 452.CrossRefPubMedGoogle Scholar
  3. 3.
    Spratt, N. T., Jr. (1963) Role of the substratum, supra cellular continuity and differential growth in morphogenetic cell movements. Dev. Biol. 7, 51–63.CrossRefPubMedGoogle Scholar
  4. 4.
    Gallera, J. and Nicolet, G. (1961) Quelques cooentaires sur les methodes de culture in vitro de jeunes blastodermes de poulet. Experientia 17, 134, 135.CrossRefPubMedGoogle Scholar
  5. 5.
    Pannett, C. A. and Compton, A. (1924) The cultivation of tissue in saline embryonic juice. Lancet 206, 381–384.CrossRefGoogle Scholar
  6. 6.
    Hamburger, V. and Hamilton, H. L. (1951) A series of normal stages in the development of the chick embryo. J. Morphol. 88, 49–67.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Amata Hornbruch
    • 1
  1. 1.Retired, MRC Centre for Developmental NeurobiologyKing's College LondonLondonUK

Personalised recommendations