As the title indicates, this culture method was developed by Denis New and first described in 1955 (1). It enables the observer to study the events of gastru-lation in the chick embryo in much greater detail than possible until then. It also opens the way to microsurgery without the problem that yolk and the vitelline membrane cause. Studying the effects of treatment with compound affecting morphogenesis and development became meaningful, because precise concentrations and volumes could be administered and successfully washed out again.
Most of the early work on the mechanics of gastrulation was performed on the egg of Xenopus laevis, because of its accessibility. New Culture offered a chance to emulate and refine some of those experiments in the chick embryo.
Several attempts have been made to culture blastoderms or fragments thereof in vitro, but none have been very successful. The chorioallantoic membrane (CAM grafts) has been used as a substratum to study...
KeywordsChick Embryo Balance Salt Solution Watch Glass Vitelline Membrane Morphogenetic Movement
- 1.New, D. A. T. (1955) A new technique for the cultivation of the chick embryo in vitro. J. Embryol. Exp. Morphol. 3, 326–331.Google Scholar