The following methods constitute a brief description of chick egg incubation and embryo harvesting. For further details concerning optimizing embryo viability during incubation, the reader is referred to the excellent work of New (1). Embryos are staged according to the staging series devised by Hamburger and Hamilton (2), which has recently been reproduced (3). The reader is also referred to Chapter 14. A further, detailed staging series for very young embryos has also been devised (4).
Howard's ringer. (0.12 M NaCl, 0.0015 M CaCl2, 0.005 M KCl (per liter: 7.2 g NaCl, 0.17 g CaCl2, 0.37 g KCl, pH 7.2, with very dilute HCl) containing penecillin— streptomycin (AAM, Gibco, Paisley, Scotland).
Pair of curved, sharp-pointed scissors.
Fine watchmakers' forceps (#5 or 55).
Pair of spring scissors (Vannas scissors).
Stainless-steel spatula, 5- to 8-mm wide tip.
Dissecting microscope with magnification to 50O× (minimum), preferably...
KeywordsStaging Series Vitelline Membrane Specific Developmental Stage Young Embryo Cold Light Source
Research in the author's laboratory is supported the MRC, BBSRC, and the Wellcome Trust; and he is a research fellow of the Leverhulme Trust.