Mouse Primordial Germ Cells

Isolation and In Vitro Culture
  • Patricia A. Labosky
  • Brigid L. M. Hogan
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

Since the original publication of this chapter on the manipulation of primordial germ cells (PGCs) into embryonic germ (EG) cell lines, the field has progressed considerably. Perhaps the most exciting advance has been the derivation of pluripotent human EG cell lines, using methods similar to those outlined here (1). Cells derived from these human EG lines can stimulate partial motor recovery in rats paralyzed by viral-induced motor neuron injury (2). This provides an exciting example of the therapeutic potential of human stem cell research. However, it is likely that the effect is indirect, due to the enhancement of host neuron survival and function by growth factors produced by the grafted cells. Another advance has been the use of EG lines to follow the process of erasure of genetic imprinting that normally occurs during the migration of PGCs to the gonad. However, caution is again needed, since EG cells grown in vitromay not accurately reflect all the imprinting...


Leukemia Inhibitory Factor Stem Cell Factor Primordial Germ Cell Secondary Culture Genital Ridge 
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Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Patricia A. Labosky
    • 1
  • Brigid L. M. Hogan
    • 2
  1. 1.Center for Stem Cell Biology, Department of Cell and Developmental BiologyVanderbilt UniversityNashvilleUSA
  2. 2.Cell and Molecular Biology ProgramDuke UniversityDurhamUSA

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